HSPG anticorps

N° du produit ABIN335359

Détail du produit anti-HSPG anticorps

Antigène: HSPG (Heparan Sulphate Protoglycans (HSPG))

Reactivité: Humain, Boeuf (Vache), Souris, Rat

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Human, mouse, rat and cow.

Purification: Purified

Immunogène: A7L6 is a rat monoclonal IgG2a antibody derived by fusion of X63 Ag8.653 mouse myeloma cells with spleen cells from a Fisher rat immunized with high molecular mass material derived from the Engelbreth-Holm-Swarm (EHS) tumor matrix containing laminin, entactin and HSPG.

Clone: A7L6

Isotype: IgG2a

Information d'application

Indications d'application: A7L6 recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment. APPLICATIONS A7L6 is useful for immunoprecipitation, immunoblotting, immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Références pour anticorps anti-HSPG (ABIN335359)

Tingbø, Kolset, Ofstad, Enersen, Hannesson: "Identification and distribution of heparan sulfate proteoglycans in the white muscle of Atlantic cod (Gadus morhua) and spotted wolffish (Anarhichas minor)." dans: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol. 143, Issue 4, pp. 441-52, (2006) (PubMed).

Tapanadechopone, Hassell, Rigatti, Couchman: "Localization of glycosaminoglycan substitution sites on domain V of mouse perlecan." dans: Biochemical and biophysical research communications, Vol. 265, Issue 3, pp. 680-90, (2000) (PubMed).

Couchman, Ljubimov, Sthanam, Horchar, Hassell: "Antibody mapping and tissue localization of globular and cysteine-rich regions of perlecan domain III." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 9, pp. 955-63, (1995) (PubMed).

Ljubimov, Bartek, Couchman, Kapuller, Veselov, Kovarik, Perevoshchikov, Krutovskikh: "Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies." dans: International journal of cancer. Journal international du cancer, Vol. 50, Issue 4, pp. 562-6, (1992) (PubMed).

Couchman, Ljubimov: "Mammalian tissue distribution of a large heparan sulfate proteoglycan detected by monoclonal antibodies." dans: Matrix (Stuttgart, Germany), Vol. 9, Issue 4, pp. 311-21, (1989) (PubMed).

Horiguchi, Couchman, Ljubimov, Yamasaki, Fine: "Distribution, ultrastructural localization, and ontogeny of the core protein of a heparan sulfate proteoglycan in human skin and other basement membranes." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 7, pp. 961-70, (1989) (PubMed).

Couchman: "Heterogeneous distribution of a basement membrane heparan sulfate proteoglycan in rat tissues." dans: The Journal of cell biology, Vol. 105, Issue 4, pp. 1901-16, (1987) (PubMed).

Détails sur HSPG

Antigène: HSPG (Heparan Sulphate Protoglycans (HSPG))

Autre désignation: Heparan Sulphate Proteoglycan (HSPG Produits)

Synonymes: anticorps CDW44, anticorps CSPG8, anticorps ECMR-III, anticorps HCELL, anticorps HUTCH-I, anticorps IN, anticorps LHR, anticorps MC56, anticorps MDU2, anticorps MDU3, anticorps MIC4, anticorps Pgp1, anticorps AU023126, anticorps AW121933, anticorps AW146109, anticorps HERMES, anticorps Ly-24, anticorps Pgp-1, anticorps CD44A, anticorps METAA, anticorps RHAMM, anticorps CD44 molecule (Indian blood group), anticorps glypican 3, anticorps CD44 antigen, anticorps CD44, anticorps GPC3, anticorps Cd44

Sujet: Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). At least two forms of basement membrane heparan sulphate proteoglycan (HSPG) have been identified. One with a large core protein (> 400 kD) and one with a small core protein (30 kD). The large HSPG is probably the most abundant basement membrane proteoglycan. It is located predominantly in the lamina lucida, where it forms clustered aggregates and interacts with other basement membrane components to form the matrix. In addition, it also plays a critical role in attachment of cells to the basal membrane via integrin receptors.