Souris anti-Humain IgG (Fab Region) Anticorps (PE)

Détails pour le produit réf. ABIN349709, Fournisseur: Connectez-vous pour afficher
Antigène
Épitope
Fab Region
5028
2578
1931
1069
1007
857
330
298
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivité
Humain
2911
2859
2405
1752
1485
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Hôte
Souris
6893
4912
1878
894
703
401
92
54
52
21
9
8
5
5
2
1
1
Clonalité (Clone)
Monoclonal ()
Conjugué
PE
2235
1889
1740
1374
765
679
519
300
298
288
274
268
217
188
178
124
103
79
69
60
54
46
43
40
40
36
34
32
29
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
16
15
15
15
15
15
14
12
12
11
10
10
9
9
9
9
9
9
9
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
Options
Fournisseur
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Supplier Product No.
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Immunogène Purified human IgG.
Clone 4A11
Isotype IgG2b
Specificité The antibody 4A11 reacts with Fab fragment of human IgG.
Attributs du produit The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Sujet Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Poids moléculaire 150 kDa
Domaine de recherche Immunology, Secondary Antibodies
Indications d'application The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 6 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Commentaires

Excitation/Emission wavelength: 496 nm/576 nm

Restrictions For Research Use only
Format Liquid
Reconstitution No reconstitution is necessary.
Concentration 0.1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Agent conservateur Sodium azide
Précaution d'utilisation WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Conseil sur la manipulation Do not freeze.
Avoid prolonged exposure to light.
Stock 4 °C
Stockage commentaire Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fab Region) antibody (PE) (ABIN349709) Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." dans: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." dans: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." dans: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." dans: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).