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GFP anticorps

GFP Reactivité: Toutes les espèces WB, IHC Hôte: Mouton Polyclonal unconjugated
N° du produit ABIN350302
  • Antigène Voir toutes GFP Anticorps
    GFP (Green Fluorescent Protein (GFP))
    Reactivité
    • 180
    • 19
    • 15
    • 15
    • 12
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Toutes les espèces
    Hôte
    • 76
    • 74
    • 35
    • 11
    • 9
    • 5
    • 5
    • 1
    Mouton
    Clonalité
    • 122
    • 89
    Polyclonal
    Conjugué
    • 105
    • 14
    • 12
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GFP est non-conjugé
    Application
    • 172
    • 87
    • 56
    • 41
    • 34
    • 33
    • 32
    • 21
    • 16
    • 14
    • 14
    • 14
    • 12
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificité
    Specific for EGFP/GFP/Venus
    Immunogène
    Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.
    Isotype
    IgG
    Top Product
    Discover our top product GFP Anticorps primaire
  • Indications d'application
    Use at a concentration of 10-50 µg/ml.
    The optimal working concentration should be determined by the end user.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute in 100 µL of MQ water. Centrifuge to remove any insoluble material.
    Conseil sur la manipulation
    Avoid freeze and thaw cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
    Date de péremption
    12 months
  • Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." dans: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

  • Antigène
    GFP (Green Fluorescent Protein (GFP))
    Abstract
    GFP Produits
    Synonymes
    anticorps green fluorescent protein, anticorps gfp
    Classe de substances
    Viral Protein
    Sujet
    GFP is a  naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: Enhanced Green Fluorescence Protein.
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