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LIMK2 anticorps (LIM Domain Kinase 2) (pSer291)

Détail du produit anti-LIMK2 anticorps No. ABIN350731, Fournisseur: Connectez-vous pour afficher
Antigène
  • xlimk2
  • zgc:91990
  • wu:fa97c09
  • wu:fk73e11
  • LIMK2
  • Limk2b
  • Link2
  • LIMK
  • 9430059K01
  • A930024P04Rik
  • C85310
  • Limk2a
  • LIM domain kinase 2
  • similar to LIM domain kinase 2
  • LIM motif-containing protein kinase 2
  • limk2
  • LIMK2
  • LOC100223808
  • Limk2
Épitope
pSer291, pSer293
28
13
10
9
8
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Souris, Rat (Rattus), Humain
126
84
79
2
1
1
1
1
1
1
1
1
Hôte
Mouton
116
6
2
2
Clonalité
Polyclonal
Conjugué
Cet anticorp LIMK2 est non-conjugé
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
112
55
44
39
22
10
3
2
1
1
1
1
Options
Fournisseur
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Immunogène A synthetic peptide from the phospho S291 & S293 region of human LIMK2 (LIM domain kinase 2, LIMK-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse.
Specificité Specific for LIMK2.
Purification Whole serum
Autre désignation LIMK2 (LIMK2 Antibody Extrait)
Sujet Function: Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subcellular location: Cytoplasm. Nucleus. Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus, and isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Tissue specificity: Highest expression in the placenta, moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. Also known as: LIM domain kinase 2, LIMK-2.
Indications d'application A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. This antibody may also recognise the unphosphorylated form.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation Avoid freeze and thaw cycles.
Stock 4 °C/-20 °C
Stockage commentaire Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Date de péremption 12 months
Background publications Sumi, Matsumoto, Nakamura: "Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase." dans: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 670-6, 2001 (PubMed).

Dunham, Shimizu, Roe, Chissoe, Hunt, Collins, Bruskiewich, Beare, Clamp, Smink, Ainscough, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beasley, Bird, Blakey, Bridgeman, Buck, Burgess, Burrill et al.: "The DNA sequence of human chromosome 22. ..." dans: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Maekawa, Ishizaki, Boku, Watanabe, Fujita, Iwamatsu, Obinata, Ohashi, Mizuno, Narumiya: "Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase." dans: Science (New York, N.Y.), Vol. 285, Issue 5429, pp. 895-8, 1999 (PubMed).

Osada, Hasada, Inazawa, Uchida, Ueda, Takahashi, Takahashi: "Subcellular localization and protein interaction of the human LIMK2 gene expressing alternative transcripts with tissue-specific regulation." dans: Biochemical and biophysical research communications, Vol. 229, Issue 2, pp. 582-9, 1997 (PubMed).

Okano, Hiraoka, Otera, Nunoue, Ohashi, Iwashita, Hirai, Mizuno: "Identification and characterization of a novel family of serine/threonine kinases containing two N-terminal LIM motifs." dans: The Journal of biological chemistry, Vol. 270, Issue 52, pp. 31321-30, 1996 (PubMed).