A synthetic peptide from the n-terminal of human SUMO4 (Small ubiquitin-related modifier4) conjugated to an immunogenic carrier protein was used as the antigen.
SUMO4
Reactivité: Humain
WB, IHC, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Conseil sur la manipulation
Avoid freeze and thaw cycles.
Stock
4 °C/-20 °C
Stockage commentaire
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Date de péremption
12 months
Antigène
SUMO4
(Small Ubiquitin Related Modifier 4 (SUMO4))
anticorps IDDM5, anticorps SMT3H4, anticorps SUMO-4, anticorps dJ281H8.4, anticorps small ubiquitin-like modifier 4, anticorps small ubiquitin-related modifier 4, anticorps SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae), anticorps SUMO4, anticorps sumo4
Sujet
Function: Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. Tissue specificity: Expressed mainly in adult and embryonic kidney. Expressed at various levels in immune tissues, with the highest expression in the lymph node and spleen. Post-translational modification: In contrast to SUMO1, SUMO2 and SUMO3, seems to be insensitive to sentrin-specific proteases due to the presence of Pro-90. This may impair processing to mature form and conjugation to substrates. Also known as: Small ubiquitin-related modifier 4, SUMO-4, Small ubiquitin-like protein 4, SUMO4, SMT3H4.