HDAC4 anticorps (C-Term)

N° du produit ABIN356644

Détail du produit anti-HDAC4 anticorps

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC4 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody is specific to HDAC4 (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogène: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC4.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western Blot: 1-100-1/500.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur HDAC4

Antigène: HDAC4 (Histone Deacetylase 4 (HDAC4))

Autre désignation: HDAC4 (HDAC4 Produits)

Synonymes: anticorps CG1770, anticorps DHDAC4, anticorps Dmel\\CG1770, anticorps GC1770, anticorps HDAC, anticorps HDAC4a, anticorps dHDAC4, anticorps dmHDA405, anticorps hdac4, anticorps dana_GLEANR_19966, anticorps DanaGF18710, anticorps GF18710, anticorps HDAC4, anticorps AHO3, anticorps BDMR, anticorps HA6116, anticorps HD4, anticorps HDAC-4, anticorps HDAC-A, anticorps HDACA, anticorps 4932408F19Rik, anticorps AI047285, anticorps wu:fa96d08, anticorps wu:fc56f08, anticorps zgc:152701, anticorps histone deacetylase 4, anticorps hypothetical protein, anticorps Histone deacetylase 4, anticorps GF18710 gene product from transcript GF18710-RC, anticorps PTRG_03699, anticorps PGTG_15512, anticorps HDAC4, anticorps Dana\GF18710, anticorps Hdac4, anticorps hdac4

Sujet: DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.Synonyms: HD4, Histone deacetylase 4, KIAA0288

ID gène: 9759, 9606

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process