Protein A Chromatography followed by two-step phosphospecific peptide affinity purification.
Immunogène
This antibody is generated from rabbits immunized with a KLH conjugated synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated Thr202 and Tyr204 of human ERK1.
MAPK3
Reactivité: Rat
WB, IHC, IF, FACS, ICC
Hôte: Lapin
Polyclonal
HRP
Indications d'application
ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Wang, Jin, Zou, Li, Cheng, Luo, Geng, Zhang: "Activation of Toll-like receptor 7 regulates the expression of IFN-λ1, p53, PTEN, VEGF, TIMP-1 and MMP-9 in pancreatic cancer cells." dans: Molecular medicine reports, Vol. 13, Issue 2, pp. 1807-12, (2016) (PubMed).
Antigène
ERK1 (MAPK3)
(Mitogen-Activated Protein Kinase 3 (MAPK3))
MAPK3 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets.Synonyms: ERK-1, ERT2, Extracellular signal-regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 3, MAP kinase isoform p44, MAPK 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, PRKM3, p44-ERK1, p44-MAPK