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GRP94 anticorps

HSP90B1 Reactivité: Poulet WB, IF, FACS, IP, ICC Hôte: Rat Monoclonal 9G10 unconjugated
N° du produit ABIN361653
  • Antigène Voir toutes GRP94 (HSP90B1) Anticorps
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Reactivité
    • 133
    • 86
    • 55
    • 39
    • 35
    • 31
    • 30
    • 30
    • 28
    • 28
    • 26
    • 26
    • 25
    • 17
    • 10
    • 2
    • 2
    • 1
    • 1
    Poulet
    Hôte
    • 93
    • 39
    • 24
    • 2
    • 1
    Rat
    Clonalité
    • 94
    • 65
    Monoclonal
    Conjugué
    • 69
    • 12
    • 11
    • 11
    • 10
    • 8
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp GRP94 est non-conjugé
    Application
    • 151
    • 82
    • 68
    • 63
    • 49
    • 45
    • 38
    • 21
    • 13
    • 13
    • 12
    • 11
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Specificité
    Detects ~98 kDa. Does not detect human HSP90, Grp74, or GrpE from E.coli.
     Réactivité croisée
    Poulet, Boeuf (Vache), Chien, Cobaye, Hamster, Cheval, Humain, Singe, Souris, Porc, Lapin, Rat, Mouton, Xenopus laevis
    Purification
    Protein G Purified
    Immunogène
    Purified Grp94 isolated from chicken oviducts
    Clone
    9G10
    Isotype
    IgG2a
    Top Product
    Discover our top product HSP90B1 Anticorps primaire
  • Indications d'application
    • WB (1:2000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    0.5 μg/ml of ABIN361653 was sufficient for detection of Grp94 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Antigène
    GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
    Autre désignation
    GRP94 (HSP90B1 Produits)
    Sujet
    Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
    ID gène
    7184
    NCBI Accession
    NP_003290
    UniProt
    P14625
    Pathways
    Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
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