DNAJB1 anticorps

N° du produit ABIN361661

Détail du produit anti-DNAJB1 anticorps

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DNAJB1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Specificité: Detects ~40 kDa. Yeast specific. Does not cross react with Human, Mouse or Rat.

Réactivité croisée: Saccharomyces cerevisiae

Purification: Protein G Purified

Immunogène: Full length protein HSP40 (YDJ1)

Clone: 2A7-H6

Isotype: IgG1 kappa

Information d'application

Indications d'application:

• WB (1:2000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN361661 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-DNAJB1 (ABIN361661)

Knighton, Nitika, Waller, Strom, Wolfgeher, Reitzel, Truman: "Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock." dans: Journal of proteomics, Vol. 206, pp. 103416, (2020) (PubMed).

Sluder, Nitika, Knighton, Truman: "The Hsp70 co-chaperone Ydj1/HDJ2 regulates ribonucleotide reductase activity." dans: PLoS genetics, Vol. 14, Issue 11, pp. e1007462, (2019) (PubMed).

Détails sur DNAJB1

Antigène: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Autre désignation: HSP40 (DNAJB1 Produits)

Synonymes: anticorps HSPF1, anticorps Hdj1, anticorps Hsp40, anticorps RSPH16B, anticorps Sis1, anticorps CG10578, anticorps DNAJ-1, anticorps DNAJ1, anticorps DROJ1, anticorps Dmel\\CG10578, anticorps DnaJ1 64EF, anticorps DroJ1, anticorps EU3500, anticorps HSP40, anticorps HSP40/HDJ1, anticorps Hsp-40, anticorps anon-WO0140519.166, anticorps anon-WO0172774.135, anticorps dHDJ1, anticorps dHDJ1/HSP40, anticorps dHdj1, anticorps dhdJ1, anticorps dhdj-1, anticorps dhdj1, anticorps dnaJ-1, anticorps droj1, anticorps hsp40, anticorps dnajb4, anticorps hdj1, anticorps hspf1, anticorps sis1, anticorps DnaJ-5, anticorps MAS5, anticorps dnaJ, anticorps 0610007I11Rik, anticorps dnajb1, anticorps zf-Hsp40, anticorps zgc:101068, anticorps DnaJ heat shock protein family (Hsp40) member B1, anticorps DnaJ-like-1, anticorps DnaJ (Hsp40) homolog 5, anticorps type I HSP40 co-chaperone YDJ1, anticorps DnaJ protein, anticorps molecular chaperone DnaJ, anticorps DnaJ (Hsp40) homolog, subfamily B, member 1a, anticorps DNAJB1, anticorps Dnajb1, anticorps DnaJ-1, anticorps dnajb1, anticorps DnaJ-5, anticorps YDJ1, anticorps dnaJ, anticorps dnajb1a

Sujet: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).

ID gène: 855661

NCBI Accession: NP_014335

UniProt: P25491