HMOX1 anticorps (AA 1-30)

N° du produit ABIN361697

Détail du produit anti-HMOX1 anticorps

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Épitope: AA 1-30

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HMOX1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: Detects 32 kDa. Does not cross-react with HO-2.

Réactivité croisée: Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat

Purification: Protein G Purified

Immunogène: Human HO-1 synthetic peptide, amino acids 1-30

Clone: 1F12-A6

Isotype: IgG1 kappa

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml was sufficient for detection of HO-1 in 10 μg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-HMOX1 (ABIN361697)

Wang, Zhang, Xia, Zhang, Chen, Yang: "Protective effect of silencing Stat1 on high glucose-induced podocytes injury via Forkhead transcription factor O1-regulated the oxidative stress response." dans: BMC molecular and cell biology, Vol. 20, Issue 1, pp. 27, (2020) (PubMed).

Kanzaki, Shinohara, Itohiya-Kasuya, Ishikawa, Nakamura: "Nrf2 activation attenuates both orthodontic tooth movement and relapse." dans: Journal of dental research, Vol. 94, Issue 6, pp. 787-94, (2015) (PubMed).

Kanzaki, Shinohara, Kajiya, Fukaya, Miyamoto, Nakamura: "Nuclear Nrf2 induction by protein transduction attenuates osteoclastogenesis." dans: Free radical biology & medicine, Vol. 77, pp. 239-48, (2014) (PubMed).

Al-Rifai, Rücker, Amslinger: "Opening or closing the lock? When reactivity is the key to biological activity." dans: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 19, Issue 45, pp. 15384-95, (2013) (PubMed).

Détails sur HMOX1

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Autre désignation: HO-1 (HMOX1 Produits)

Synonymes: anticorps HMOX1, anticorps ARABIDOPSIS THALIANA HEME OXYGENASE 1, anticorps ATHO1, anticorps F18A8.4, anticorps F18A8_4, anticorps GENOMES UNCOUPLED 2, anticorps GUN2, anticorps HEME OXYGENASE, anticorps HEME OXYGENASE 1, anticorps HEME OXYGENASE 6, anticorps HO1, anticorps HY1, anticorps HY6, anticorps PLASTID HEME OXYGENASE, anticorps REVERSAL OF THE DET PHENOTYPE 4, anticorps HO-1, anticorps wu:fc27c04, anticorps zgc:65984, anticorps D8Wsu38e, anticorps Hemox, anticorps Hmox, anticorps Hsp32, anticorps HEOXG, anticorps Heox, anticorps Ho-1, anticorps Ho1, anticorps hsp32, anticorps MGC132176, anticorps Hmox1, anticorps HMOX1D, anticorps HSP32, anticorps bK286B10, anticorps heme oxygenase 1, anticorps Plant heme oxygenase (decyclizing) family protein, anticorps Heme oxygenase, anticorps heme oxygenase, anticorps heme oxygenase 1, chloroplastic, anticorps heme oxygenase 1a, anticorps heme oxygenase 1 L homeolog, anticorps HMOX1, anticorps TED4, anticorps ho1, anticorps P9301_RS17555, anticorps A9601_RS17590, anticorps MAE_RS06565, anticorps HO1, anticorps hmox1a, anticorps CPE0214, anticorps Cyan7425_2962, anticorps Hmox1, anticorps hmox1.L, anticorps AM1_C0205, anticorps CC1G_11686, anticorps CpipJ_CPIJ007246

Sujet: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin (1). These products have important physiological effects as carbon monoxide is a potent vasodilator, biliverdin and bilirubin are potent antioxidants, and the free iron increases oxidative stress and regulates the expression of many mRNAs (2). There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3, however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals (3). HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation (4). It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency (5), susceptibility to atherosclerotic lesion formation (6), endothelial cell injury, and growth retardation (7). Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress (4).

ID gène: 3162

NCBI Accession: NP_002124

UniProt: P09601

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome