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HSP90AB1 anticorps (AA 185-335)

HSP90AB1 Reactivité: Humain WB, IHC, ELISA, IP Hôte: Souris Monoclonal Hyb-K3701 unconjugated
N° du produit ABIN361720
  • Antigène Voir toutes HSP90AB1 Anticorps
    HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
    Épitope
    • 16
    • 13
    • 10
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 185-335
    Reactivité
    • 137
    • 91
    • 80
    • 15
    • 15
    • 14
    • 11
    • 9
    • 8
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 107
    • 29
    • 1
    Souris
    Clonalité
    • 104
    • 33
    Monoclonal
    Conjugué
    • 75
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HSP90AB1 est non-conjugé
    Application
    • 110
    • 51
    • 49
    • 38
    • 24
    • 24
    • 23
    • 23
    • 15
    • 14
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Specificité
    Detects 90 kDa. This is a beta specific product, does not cross-react with alpha isoforms.
     Réactivité croisée
    Humain, Souris
    Purification
    Protein G Purified
    Immunogène
    Recombinant human HSP90beta, Specificity mapped to amino acids 185-335
    Clone
    Hyb-K3701
    Isotype
    IgM
    Top Product
    Discover our top product HSP90AB1 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • IHC (1:3000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    1 μg/ml was sufficient for detection of HSP90β in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Muralidharan, Ambade, Fulham, Deshpande, Catalano, Mandrekar: "Moderate alcohol induces stress proteins HSF1 and hsp70 and inhibits proinflammatory cytokines resulting in endotoxin tolerance." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 4, pp. 1975-87, (2014) (PubMed).

    Bates, Salzman, May, Garcia, Hogan, McIntosh, Schlissel, Brown: "Extensive gene-specific translational reprogramming in a model of B cell differentiation and Abl-dependent transformation." dans: PLoS ONE, Vol. 7, Issue 5, pp. e37108, (2012) (PubMed).

  • Antigène
    HSP90AB1 (Heat Shock Protein 90kDa alpha (Cytosolic), Class B Member 1 (HSP90AB1))
    Autre désignation
    HSP90 beta (HSP90AB1 Produits)
    Synonymes
    anticorps D6S182, anticorps HSP84, anticorps HSP90B, anticorps HSPC2, anticorps HSPCB, anticorps GRP94, anticorps TRA1, anticorps hsp90b, anticorps 90kDa, anticorps AL022974, anticorps C81438, anticorps Hsp84, anticorps Hsp84-1, anticorps Hsp90, anticorps Hspcb, anticorps Hsp70, anticorps Hsp70-1, anticorps Hsp70.1, anticorps hsp68, anticorps HSP90-BETA, anticorps hsp90beta, anticorps wu:fa29f01, anticorps wu:fa91e11, anticorps wu:fd59e11, anticorps wu:gcd22h07, anticorps HSP90, anticorps heat shock protein 90 alpha family class B member 1, anticorps heat shock protein 90 beta family member 1, anticorps Heat Shock Protein 90, endoplasmic reticulum, anticorps heat shock protein 90B, anticorps heat shock protein 90 alpha (cytosolic), class B member 1, anticorps heat shock protein 1B, anticorps heat shock protein 90kDa alpha family class B member 1 S homeolog, anticorps heat shock protein 90, alpha (cytosolic), class B member 1, anticorps HSP90AB1, anticorps HSP90B1, anticorps HSP90B, anticorps hsp90ab1, anticorps Hsp90ab1, anticorps Hspa1b, anticorps hsp90ab1.S
    Sujet
    HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms α and β, which share 85 % sequence amino acid homology. The two isoforms of HSP90 are expressed in the cytosolic compartment (1). Despite the similarities, HSP90α exists predominantly as a homodimer while HSP90β exists mainly as a monomer (2). From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (3-6). Furthermore, HSP90 is highly conserved between species, having 60 % and 78 % amino acid similarity between mammalian and the corresponding yeast and Drosophila proteins, respectively. HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (7-8). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (9). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
    ID gène
    3326
    NCBI Accession
    NP_031381
    UniProt
    P08238
    Pathways
    Regulation of Cell Size
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