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HSP90 anticorps

HSP90 Reactivité: Humain IP, IF, ICC Hôte: Souris Monoclonal 8D3 unconjugated
N° du produit ABIN361731
  • Antigène Voir toutes HSP90 Anticorps
    HSP90 (Heat Shock Protein 90 (HSP90))
    Reactivité
    • 121
    • 63
    • 63
    • 33
    • 26
    • 21
    • 20
    • 20
    • 12
    • 10
    • 10
    • 10
    • 10
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 97
    • 66
    • 3
    • 1
    Souris
    Clonalité
    • 100
    • 67
    Monoclonal
    Conjugué
    • 58
    • 12
    • 11
    • 11
    • 11
    • 11
    • 10
    • 10
    • 9
    • 9
    • 7
    • 4
    • 4
    Cet anticorp HSP90 est non-conjugé
    Application
    • 139
    • 104
    • 93
    • 70
    • 61
    • 57
    • 33
    • 20
    • 13
    • 10
    • 2
    • 2
    • 1
    Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificité
    Detects 90 kDa. Co-immunoprecipitates HSP90 complexes, including HSP70, Hop, Ah receptors, glucocorticoid receptors, heme-regulated eukaryotic initiation factor 2α (eIF-2α) kinase (HRI).
     Réactivité croisée
    Humain, Souris, Lapin, Rat
    Purification
    PEG Purified
    Immunogène
    Ah receptor (Aryl hydrocarbon receptor)
    Clone
    8D3
    Isotype
    IgM
    Top Product
    Discover our top product HSP90 Anticorps primaire
  • Indications d'application
    • ICC/IF (1:100)
    • IP (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    Goat anti-mouse IgM was used to bind 25 μl of protein G-Sepharose. ABIN361731 IgM from 0.5 ml of high speed supernatant medium was loaded onto the IgG resin and incubated with 100 μl of rabbit reticulocyte lysate for 30 min. at 30C. After washing (4X1 ml), bound proteins were resolved on SDS PAGE, including HSP90, HSP70 and Hop.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Shimamura, Perera, Foley, Sang, Rodig, Inoue, Chen, Li, Carretero, Li, Sinha, Carey, Borgman, Jimenez, Meyerson, Ying, Barsoum, Wong, Shapiro: "Ganetespib (STA-9090), a nongeldanamycin HSP90 inhibitor, has potent antitumor activity in in vitro and in vivo models of non-small cell lung cancer." dans: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 18, Issue 18, pp. 4973-85, (2012) (PubMed).

    Ardi, Alexander, Johnson, McAlpine: "Macrocycles that inhibit the binding between heat shock protein 90 and TPR-containing proteins." dans: ACS chemical biology, Vol. 6, Issue 12, pp. 1357-66, (2011) (PubMed).

  • Antigène
    HSP90 (Heat Shock Protein 90 (HSP90))
    Autre désignation
    HSP90 (HSP90 Produits)
    Synonymes
    anticorps EL52, anticorps HSP86, anticorps HSP89A, anticorps HSP90A, anticorps HSP90N, anticorps HSPC1, anticorps HSPCA, anticorps HSPCAL1, anticorps HSPCAL4, anticorps HSPN, anticorps Hsp89, anticorps Hsp90, anticorps LAP2, anticorps git10, anticorps swo1, anticorps HSP90, anticorps htpG, anticorps SCBAC25F8.08, anticorps 23.m06066, anticorps 17.m07646, anticorps HSP90-1, anticorps 143198_at, anticorps 83, anticorps 83K HSP, anticorps DMHSP82, anticorps E(sev)3A, anticorps E(sina)2, anticorps HSP82, anticorps HSP83, anticorps ORF1, anticorps Su(Raf)3A, anticorps anon-EST:Liang-2.53, anticorps anon-WO0068693, anticorps anon-WO0140519.209, anticorps clone 2.53, anticorps en(lz)3C/4C, anticorps hsp84, anticorps l(3)j5C2, anticorps ms(3)08445, anticorps stc, anticorps DmelCG1242, anticorps CG1242, anticorps Hsp86, anticorps Hspca, anticorps 86kDa, anticorps 89kDa, anticorps AL024080, anticorps AL024147, anticorps Hsp86-1, anticorps hsp4, anticorps hsp86, anticorps hsp89, anticorps hsp90, anticorps hsp90a, anticorps hspc1, anticorps hspca, anticorps hspn, anticorps lap2, anticorps Hsp90alpha, anticorps heat shock protein 90 alpha family class A member 1, anticorps heat shock protein Hsp90, anticorps Hsp90 chaperone, anticorps Heat shock protein 90, anticorps heat shock protein 90, anticorps chaperone protein HtpG, anticorps molecular chaperone HtpG, anticorps Heat Shock Protein 90, anticorps uncharacterized LOC100384473, anticorps Heat shock protein 83, anticorps heat shock protein 90, alpha (cytosolic), class A member 1, anticorps heat shock protein 90 alpha family class B member 1, anticorps heat shock protein 90kDa alpha family class A member 1 L homeolog, anticorps heat shock protein HSP 90-alpha, anticorps HSP90AA1, anticorps hsp90, anticorps HSP90, anticorps daf-21, anticorps htpG, anticorps SCO7516, anticorps MAP_RS10510, anticorps TP04_0646, anticorps TP01_0934, anticorps APH_RS03525, anticorps GbCGDNIH1_0315, anticorps MAV_2118, anticorps HSP90C, anticorps BBOV_IV008400, anticorps BBOV_III007380, anticorps ACICU_00312, anticorps ECL_01244, anticorps YE105_C1172, anticorps pco153543(105), anticorps Hsp83, anticorps Hsp90aa1, anticorps HSP90AB1, anticorps hsp90aa1.1.L, anticorps LOC108698781
    Sujet
    HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase (5). When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). For more information visit our HSP90 Scientific Resource Guide at http://www.HSP90.ca.
    ID gène
    3326
    NCBI Accession
    NP_031381
    UniProt
    P08238
    Pathways
    M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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