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HSP70 anticorps

HSP70 Reactivité: Humain WB, IHC, IF, IP, ICC, AA Hôte: Souris Monoclonal 3A3 unconjugated
N° du produit ABIN361737
  • Antigène Voir toutes HSP70 Anticorps
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reactivité
    • 323
    • 199
    • 165
    • 71
    • 62
    • 53
    • 52
    • 52
    • 36
    • 33
    • 29
    • 29
    • 28
    • 23
    • 20
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    • 15
    • 13
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 206
    • 150
    • 13
    • 11
    • 11
    Souris
    Clonalité
    • 228
    • 162
    Monoclonal
    Conjugué
    • 112
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HSP70 est non-conjugé
    Application
    • 374
    • 159
    • 142
    • 122
    • 122
    • 110
    • 101
    • 66
    • 62
    • 61
    • 41
    • 33
    • 22
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    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Antibody Array (AA)
    Specificité
    Detects ~70 kDa. May detect HSP70, HSC70, p75 and HSP72.
     Réactivité croisée
    Amphibien, Bactéries, Brine Shrimp, Poulet, Drosophila melanogaster, Poisson, Humain, Souris, Rat, Saccharomyces cerevisiae
    Purification
    Protein G Purified
    Immunogène
    Human recombinant HSP70 overexpressed in E.coli
    Clone
    3A3
    Isotype
    IgG1
    Top Product
    Discover our top product HSP70 Anticorps primaire
  • Indications d'application
    • WB (1:5000)
    • ICC/IF (1:500)
    • IP (2 μg)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    0.2 μg/ml of ABIN361737 was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Paciello, Silipo, Lembo-Fazio, Curcurù, Zumsteg, Noël, Ciancarella, Sturiale, Molinaro, Bernardini: "Intracellular Shigella remodels its LPS to dampen the innate immune recognition and evade inflammasome activation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 46, pp. E4345-54, (2013) (PubMed).

  • Antigène
    HSP70 (Heat Shock Protein 70 (HSP70))
    Autre désignation
    HSP70 (HSP70 Produits)
    Synonymes
    anticorps APG-2, anticorps HS24/P52, anticorps HSPH2, anticorps RY, anticorps hsp70, anticorps hsp70RY, anticorps CG31354, anticorps HSP70, anticorps Hsp70Bb, anticorps hsp70B, anticorps hsp70Bb-prime, anticorps DmelCG5834, anticorps CG5834, anticorps HSPA1, anticorps HSP70B', anticorps HSPA6, anticorps ARABIDOPSIS HEAT SHOCK PROTEIN 70, anticorps ATHSP70, anticorps heat shock protein 70, anticorps LOC100305036, anticorps hsc70, anticorps Hsp70, anticorps Hsp70-1, anticorps Hsp70.1, anticorps hsp68, anticorps Hsp110, anticorps irp94, anticorps HSP70-2, anticorps HSPA1B, anticorps HSPA2, anticorps hsp70-5, anticorps HSP70-1, anticorps HSP70.1, anticorps HSP70.2, anticorps heat shock protein family A (Hsp70) member 4, anticorps CG5834 gene product from transcript CG5834-RA, anticorps heat shock protein 70, anticorps heat shock protein family A (Hsp70) member 6, anticorps heat shock 70kDa protein 2, anticorps heat shock 70 kD protein cognate, anticorps Hsp70 family chaperone, anticorps Heat shock protein 70, anticorps Heat shock protein 70, putative, anticorps heat shock protein 1B, anticorps heat shock protein family A member 4, anticorps heat shock 70kDa protein 1A, anticorps heat shock protein 1, anticorps Heat Shock Protein, anticorps heat shock cognate 70-kd protein, anticorps Heat shock 70 kDa protein 1A, anticorps HSPA4, anticorps Hsp70Bbb, anticorps HSP70, anticorps HSPA6, anticorps HSPA2, anticorps PCC7424_2419, anticorps Isop_1041, anticorps CGB_C3390W, anticorps Bacsa_1698, anticorps dnaK-B, anticorps LOC100305036, anticorps Hspa1b, anticorps Hspa4, anticorps HSPA1A, anticorps hsp1, anticorps hsp-70, anticorps hsp70, anticorps LOC108348108
    Sujet
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
    ID gène
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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