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PUMA anticorps (N-Term)

BBC3 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN361754
  • Antigène Voir toutes PUMA (BBC3) Anticorps
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Épitope
    • 21
    • 17
    • 16
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 113
    • 40
    • 39
    • 16
    Humain
    Hôte
    • 109
    • 7
    • 1
    Lapin
    Clonalité
    • 98
    • 19
    Polyclonal
    Conjugué
    • 52
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PUMA est non-conjugé
    Application
    • 98
    • 39
    • 39
    • 23
    • 22
    • 17
    • 16
    • 14
    • 13
    • 9
    • 5
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificité
    Detects the N-terminal domain of PUMA ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
     Réactivité croisée
    Poisson, Humain, Truite arc-en-ciel, Rat, Salmon
    Purification
    Protein A Purified
    Immunogène
    N-terminal amino acids of human PUMA
    Top Product
    Discover our top product BBC3 Anticorps primaire
  • Indications d'application
    • WB (1:500)
    • IHC (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    2 μg/ml of ABIN361753 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS, 0.02 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    -20°C
  • Wakabayashi, Kosaka, Mori, Yamada: "Prolonged expression of Puma in cholinergic amacrine cells during the development of rat retina." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 60, Issue 10, pp. 777-88, (2012) (PubMed).

  • Antigène
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Autre désignation
    PUMA (BBC3 Produits)
    Synonymes
    anticorps BBC3, anticorps Puma, anticorps JFY-1, anticorps JFY1, anticorps PUMA, anticorps PUMA/JFY1, anticorps BCL2 binding component 3, anticorps Bcl-2 binding component 3, anticorps BBC3, anticorps Bbc3, anticorps bbc3
    Sujet
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
    ID gène
    27113
    NCBI Accession
    NP_001120712
    UniProt
    Q9BXH1
    Pathways
    Signalisation p53, Positive Regulation of Endopeptidase Activity
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