Lysine (lys) (acetylated) anticorps

N° du produit ABIN361802

Détail du produit

Antigène: Lysine (lys)

Épitope: acetylated

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Immunohistochemistry (IHC)

Specificité: Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues.

Purification: Protein G Purified

Immunogène: Acetylated KLH

Clone: 7F8

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg of ABIN361802 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 μg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détail du antigène

Antigène: Lysine (lys)

Autre désignation: Lysine

Synonymes: anticorps lysine, anticorps lys

Classe de substances: Amino Acid

Sujet: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).