PDIA3 anticorps

N° du produit ABIN361815

Détail du produit anti-PDIA3 anticorps

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDIA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Detects ~57 kDa.

Réactivité croisée: Boeuf (Vache), Chien, Cobaye, Hamster, Humain, Singe, Souris, Porc, Lapin, Rat

Purification: Protein G Purified

Immunogène: Human recombinant ERp57 (Grp58)

Clone: Map-ERp57

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

0.5 μg/ml of ABIN361814 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-PDIA3 (ABIN361815)

Ortiz-Sandoval, Hughes, Dacks, Simmen: "Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins." dans: Cellular logistics, Vol. 4, Issue 4, pp. e986399, (2015) (PubMed).

Lynes, Raturi, Shenkman, Ortiz Sandoval, Yap, Wu, Janowicz, Myhill, Benson, Campbell, Berthiaume, Lederkremer, Simmen: "Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling." dans: Journal of cell science, Vol. 126, Issue Pt 17, pp. 3893-903, (2013) (PubMed).

Détails sur PDIA3

Antigène: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Autre désignation: ERp57 (PDIA3 Produits)

Synonymes: anticorps ER60, anticorps ERp57, anticorps ERp60, anticorps ERp61, anticorps GRP57, anticorps GRP58, anticorps HsT17083, anticorps P58, anticorps PI-PLC, anticorps grp-58, anticorps 58kDa, anticorps Erp, anticorps Grp58, anticorps PDI, anticorps PDI-Q2, anticorps PLC[a], anticorps Plca, anticorps 1, anticorps 25D3-membrane-associated, anticorps sb:cb825, anticorps erp57, anticorps grp58, anticorps pdia3, anticorps protein disulfide isomerase family A member 3, anticorps protein disulfide-isomerase A3, anticorps protein disulfide isomerase associated 3, anticorps protein disulfide isomerase family A, member 3, anticorps protein disulfide isomerase family A member 3 S homeolog, anticorps PDIA3, anticorps Tsp_10062, anticorps Pdia3, anticorps pdia3, anticorps pdia3.S

Sujet: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

ID gène: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis