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Phosphotyrosine anticorps (Biotin)

WB, IP, IF, IHC, ELISA, ICC Hôte: Lapin Polyclonal Biotin
N° du produit ABIN361844
  • Antigène Tous les produits Phosphotyrosine
    Phosphotyrosine
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 97
    • 27
    • 1
    Lapin
    Clonalité
    • 109
    • 16
    Polyclonal
    Conjugué
    • 45
    • 15
    • 12
    • 8
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Phosphotyrosine est conjugé à/à la Biotin
    Application
    • 88
    • 64
    • 41
    • 40
    • 39
    • 27
    • 25
    • 17
    • 13
    • 9
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
    Specificité
    Detects proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
    Purification
    Peptide Affinity Purified
    Immunogène
    Phosphotyrosine conjugated to KLH
  • Indications d'application
    • WB (1:500)
    • IHC (1:100)
    • ICC/IF (1:50)
    • ELISA (1:2000)
    • IP (1:100)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:250 dilution of ABIN361844 was sufficient for detection of tyrosine-phosphorylated species in mouse spleen lysates in western blot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS pH 7.0, 50 % glycerol, 0.01 % sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    Phosphotyrosine
    Abstract
    Phosphotyrosine Produits
    Classe de substances
    Amino Acid
    Sujet
    Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4). In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (6).
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