HSPB8 anticorps

N° du produit ABIN361852

Détail du produit anti-HSPB8 anticorps

Antigène: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSPB8 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificité: Detects ~22 kDa. Does not cross-react with HSP27 or alpha-crystallin.

Réactivité croisée: Humain, Souris, Rat

Purification: Peptide Affinity Purified

Immunogène: Human HSP22

Information d'application

Indications d'application:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:1000 dilution of ABIN361851 was sufficient for detection of HSP22 in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Références pour anticorps anti-HSPB8 (ABIN361852)

Webster, Darling, Sanders, Blazier, Vidal-Aguiar, Beaulieu-Abdelahad, Plemmons, Hill, Uversky, Bickford, Dickey, Blair: "Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels." dans: International journal of molecular sciences, Vol. 21, Issue 15, (2020) (PubMed).

Détails sur HSPB8

Antigène: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Autre désignation: HSP22 (HSPB8 Produits)

Synonymes: anticorps CMT2L, anticorps DHMN2, anticorps E2IG1, anticorps H11, anticorps HMN2, anticorps HMN2A, anticorps HSP22, anticorps MGC64408, anticorps hsc70, anticorps wu:fb01g06, anticorps wu:fi48b06, anticorps fc09c11, anticorps wu:fc04b04, anticorps wu:fc09c11, anticorps zgc:64202, anticorps AU018630, anticorps AW413033, anticorps Cryac, anticorps D5Ucla4, anticorps H11K, anticorps HSP20-like, anticorps Hsp22, anticorps heat shock protein family B (small) member 8, anticorps heat shock protein family B (small) member 8 L homeolog, anticorps heat shock protein 8, anticorps heat shock protein b8, anticorps HSPB8, anticorps hspb8.L, anticorps hspa8, anticorps hspb8, anticorps Hspb8

Sujet: HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

ID gène: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1