PDPK1 anticorps (3-phosphoinositide Dependent Protein Kinase-1) (pSer241)

Details for Product anti-PDPK1 Antibody No. ABIN361882, Fournisseur: Connectez-vous pour afficher
Antigène
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Épitope
pSer241
58
35
32
27
27
26
18
17
15
15
15
15
7
7
6
6
6
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
464
220
220
19
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Hôte
Lapin
383
73
16
Clonalité
Polyclonal
Conjugué
Cet anticorp PDPK1 est non-conjugé
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
313
158
101
91
67
65
40
31
22
10
7
5
3
2
1
Options
Fournisseur
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Supplier Product No.
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Immunogène Peptide sequence around phosphorylation site of pSer241 (A-N-S (p) -F-V) derived from Human PDK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificité The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Autre désignation PDK1 (PDPK1 Antibody Extrait)
Sujet Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.
Poids moléculaire 63 kDa
NCBI Accession NP_002604
UniProt O15530
Domaine de recherche Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Indications d'application Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Produit citée dans: Han, Quirion, Zheng: "WITHDRAWN: Glutamate Attenuates IGF-1 Receptor Signaling via NR2B containing NMDA Receptors 3 and neuronal Nitric Oxide Synthase." dans: Biochemical and biophysical research communications, 2011 (PubMed).

Liu, Huang, Chen, Zeng, Guo, Zhang, Liu, Xu, Mo, Li: "Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma." dans: Cancer, Vol. 117, Issue 16, pp. 3763-73, 2011 (PubMed).

Background publications Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." dans: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005 (PubMed).

Lim, Kikani, Wick, Dong: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003 (PubMed).

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." dans: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).

Chen, Nystrom, Dong, Li, Song, Liu, Quon: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." dans: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001 (PubMed).

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