tau anticorps (pThr231)

N° du produit ABIN362077

Cet anticorps Lapin Polyclonal détecte spécifiquement tau dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 2+ publications.

Aperçu rapide pour tau anticorps (pThr231) (ABIN362077)

Antigène: tau (tau Protein)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp tau est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-tau anticorps

Épitope: pThr231

Specificité: The antibody detects endogenous level of Tau only when phosphorylated at threonine 231.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pThr231 (V-R-T (p) -P-P) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-tau (ABIN362077)

Sun, Wei, Xiong, Wang, Xie, Wang, Yang, Wang, Lu, Liu, Wang: "Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." dans: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).

Chen, Xiong, Kong, Qu, Wang, Chen, Wang, Zhu: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." dans: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, (2011) (PubMed).

Détails sur tau

Antigène: tau (tau Protein)

Autre désignation: Tau

Sujet: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Poids moléculaire: 78 kDa

UniProt: P10636