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CHEK2 anticorps (pSer516)

CHEK2 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN362142
  • Antigène Voir toutes CHEK2 Anticorps
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Épitope
    • 30
    • 28
    • 21
    • 17
    • 16
    • 15
    • 15
    • 15
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer516
    Reactivité
    • 262
    • 97
    • 83
    • 16
    • 14
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Hôte
    • 242
    • 21
    • 1
    • 1
    Lapin
    Clonalité
    • 228
    • 37
    Polyclonal
    Conjugué
    • 117
    • 14
    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Cet anticorp CHEK2 est non-conjugé
    Application
    • 207
    • 80
    • 79
    • 72
    • 37
    • 37
    • 33
    • 28
    • 28
    • 19
    • 18
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificité
    The antibody detects endogenous level of Chk2 only when phosphorylated at serine 516.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogène
    Peptide sequence around phosphorylation site of pSer516 (Q-P-S (p) -T-S) derived from Human Chk2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
    Top Product
    Discover our top product CHEK2 Anticorps primaire
  • Indications d'application
    Western blotting: 1:500-1:1000  
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Antigène
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Autre désignation
    Chk2 (CHEK2 Produits)
    Synonymes
    anticorps CDS1, anticorps CHK2, anticorps HuCds1, anticorps LFS2, anticorps PP1425, anticorps RAD53, anticorps hCds1, anticorps fa66f08, anticorps wu:fa66f08, anticorps zgc:55865, anticorps Cds1, anticorps HUCDS1, anticorps Rad53, anticorps Chk2, anticorps cds1, anticorps chek2, anticorps chk2, anticorps hucds1, anticorps lfs2, anticorps pp1425, anticorps rad53, anticorps checkpoint kinase 2, anticorps serine/threonine-protein kinase chk2, anticorps checkpoint kinase 2 L homeolog, anticorps CHEK2, anticorps chek2, anticorps Chek2, anticorps MCYG_07308, anticorps chek2.L
    Sujet
    In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
    Poids moléculaire
    62 kDa
    NCBI Accession
    NP_001005
    UniProt
    O96017
    Pathways
    Signalisation p53, Apoptose, Cycle Cellulaire
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