EIF2A anticorps (pSer49)

N° du produit ABIN362446

Détail du produit anti-EIF2A anticorps

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Épitope: pSer49

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF2A est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificité: The antibody detects endogenous level of eIF2a only when phosphorylated at Serine 49.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: Peptide sequence around phosphorylation site of pSer49 (L-L-S (p) -E-L) derived from Human eIF2a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Détails sur EIF2A

Antigène: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Autre désignation: eIF2alpha (EIF2A Produits)

Synonymes: anticorps ATEIF2-A2, anticorps EIF2-A2, anticorps eukaryotic translation initiation factor 2 alpha subunit, anticorps EIF-2A, anticorps MST089, anticorps MSTP004, anticorps MSTP089, anticorps D030048D22, anticorps D3Ertd194e, anticorps im:7145971, anticorps zgc:110460, anticorps DDBDRAFT_0185936, anticorps DDBDRAFT_0234177, anticorps DDB_0185936, anticorps DDB_0234177, anticorps Eif2a, anticorps eif2a, anticorps eif2alpha, anticorps EIF2A, anticorps CG9946, anticorps Dmel\\CG9946, anticorps Eif2alpha, anticorps IF2A_DROME, anticorps M(1)14C, anticorps M(1)19-153, anticorps M(1)8e3-10, anticorps deIF2alpha, anticorps eIF-2, anticorps eIF-2 a, anticorps eIF-2 alpha, anticorps eIF2 alpha, anticorps eIF2-alpha, anticorps eIF2a, anticorps eIF2alpha, anticorps eiF-2alpha, anticorps elF2alpha, anticorps l(1)14Cf, anticorps l(1)19-153, anticorps eukaryotic translation initiation factor 2 alpha subunit, anticorps eukaryotic translation initiation factor 2A, anticorps eukaryotic translation initiation factor 2A, 65kDa, anticorps eukaryotic translation initiation factor 2A S homeolog, anticorps eukaryotic translation initiation factor, anticorps eukaryotic translation Initiation Factor 2alpha, anticorps EIF2 ALPHA, anticorps EIF2A, anticorps Eif2a, anticorps eif2a, anticorps eIF2a, anticorps eif2a.S, anticorps eif2A, anticorps eIF-2alpha

Sujet: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Poids moléculaire: 38 kDa

NCBI Accession: NP_004085

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly