STAT1 anticorps (AA 725-729)

N° du produit ABIN362803

Cet anticorps Lapin Polyclonal détecte spécifiquement STAT1 dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.Il a été cité dans 1 publication.

Aperçu rapide pour STAT1 anticorps (AA 725-729) (ABIN362803)

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-STAT1 anticorps

Épitope: AA 725-729

Specificité: The antibody detects endogenous level of total STAT1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogène: Peptide sequence around AA 725-729 (P-M-S-P-E) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Références pour anticorps anti-STAT1 (ABIN362803)

Cheng, Wang, Xia, Yan, Tang, Chen, Zhang, Liu, Kong, Sjöberg, Folco, Libby, Liao, Shi: "A guanidine-rich regulatory oligodeoxynucleotide improves type-2 diabetes in obese mice by blocking T-cell differentiation." dans: EMBO molecular medicine, Vol. 4, Issue 10, pp. 1112-25, (2012) (PubMed).

Détails sur STAT1

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: STAT1

Sujet: Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Poids moléculaire: 91 kDa

UniProt: P42224

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events