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Souris anti-Humain IgE (Fc Region) Anticorps

ELISA, WB, FACS, Func Monoclonal BSW17-NBS01 IgG1 kappa unconjugated
N° du produit ABIN363708
  • Antigène Tous les produits IgE
    IgE
    Épitope
    • 30
    • 19
    • 17
    • 1
    • 1
    Fc Region
    Reactivité
    • 51
    • 31
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 58
    • 21
    • 11
    • 10
    • 4
    • 1
    Souris
    Clonalité
    • 66
    • 32
    Monoclonal
    Application
    • 55
    • 40
    • 30
    • 22
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Specificité
    Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
    Attributs du produit
    IGHE, chromosome 14q32.33
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Pureté
    > 95 %, tested by analytical Gel Permeation Chromatography
    Stérilité
    Sterile filtered
    Immunogène
    purified human myeloma IgE
    Clone
    BSW17-NBS01
    Isotype
    IgG1 kappa
  • Indications d'application
    For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, sterile filtered, contains no NaN3
    Agent conservateur
    Azide free
    Conseil sur la manipulation
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    Stock
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • Antigène
    IgE
    Abstract
    IgE Produits
    Sujet
    The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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