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DARPP32 anticorps (N-Term)

PPP1R1B Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Poulet, Xenopus laevis, Poisson zèbre (Danio rerio) WB, IF, IHC (fro) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN372605
  • Antigène Voir toutes DARPP32 (PPP1R1B) Anticorps
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Épitope
    • 33
    • 17
    • 15
    • 8
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivité
    • 95
    • 83
    • 75
    • 12
    • 11
    • 11
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Chien, Poulet, Xenopus laevis, Poisson zèbre (Danio rerio)
    Hôte
    • 108
    • 12
    • 1
    • 1
    • 1
    • 1
    Lapin
    Clonalité
    • 102
    • 22
    Polyclonal
    Conjugué
    • 73
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Cet anticorp DARPP32 est non-conjugé
    Application
    • 94
    • 38
    • 35
    • 28
    • 27
    • 18
    • 17
    • 7
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    Specific for the ~32k DARPP-32 protein.
    Réactivité croisée (Details)
    Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse, Xenopus and Zebrafish.
    Species reactivity (tested):Rat.
    Purification
    Affinity Chromatography.
    Immunogène
    Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
    Isotype
    IgG
    Top Product
    Discover our top product PPP1R1B Anticorps primaire
  • Indications d'application
    Western Blot: 1/1000. Immunohistochemistry: 1/250.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Store the antibody undiluted (in aliquots) at-20 °C.
  • Bonito-Oliva, DuPont, Madjid, Ögren, Fisone: "Involvement of the Striatal Medium Spiny Neurons of the Direct Pathway in the Motor Stimulant Effects of Phencyclidine." dans: The international journal of neuropsychopharmacology, (2016) (PubMed).

    Gulati, Cheung, Antrobus, Church, Harding, Tung, Rimmington, Ma, Ron, Lehner, Ashcroft, Cox, Coll, ORahilly, Yeo: "Role for the obesity-related FTO gene in the cellular sensing of amino acids." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 7, pp. 2557-62, (2013) (PubMed).

    Rewal, Jurd, Gill, He, Ron, Janak: "Alpha4-containing GABAA receptors in the nucleus accumbens mediate moderate intake of alcohol." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 2, pp. 543-9, (2009) (PubMed).

    Haycock, Becker, Ang, Furukawa, Hornykiewicz, Kish: "Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum." dans: Journal of neurochemistry, Vol. 87, Issue 3, pp. 574-85, (2003) (PubMed).

    Waymire, Haycock: "Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells." dans: Journal of neurochemistry, Vol. 81, Issue 3, pp. 589-93, (2002) (PubMed).

    Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." dans: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

    Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." dans: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

    Zhu, Klimek, Dilley, Haycock, Stockmeier, Overholser, Meltzer, Ordway: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." dans: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, (1999) (PubMed).

  • Antigène
    DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
    Autre désignation
    DARPP32 (PPP1R1B Produits)
    Synonymes
    anticorps darpp32, anticorps darpp-32, anticorps Darpp-32, anticorps Darpp32, anticorps DARPP-32, anticorps DARPP32, anticorps AU040756, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 1B, anticorps protein phosphatase 1 regulatory inhibitor subunit 1B S homeolog, anticorps ppp1r1b, anticorps PPP1R1B, anticorps Ppp1r1b, anticorps ppp1r1b.S
    Sujet
    DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
    ID gène
    84152
    NCBI Accession
    NP_001229393
    UniProt
    Q9UD71
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