PYCARD anticorps (C-Term)

N° du produit ABIN374154

Détail du produit anti-PYCARD anticorps

Antigène: PYCARD (PYD and CARD Domain Containing (PYCARD))

Épitope: C-Term

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PYCARD est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-RESQSYLVED LERS

Specificité: This antibody is expected to recognize all reported Human isoforms of PYCARD according to NP_037390.2 and NP_660183.1.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_037390.2, NP_660183.1 Genename: PYCARD

Information d'application

Indications d'application: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx.22 kDa band observed in Hela and Human Kidney lysates(predicted MW of 22 kDa according to NP_037390.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PYCARD

Antigène: PYCARD (PYD and CARD Domain Containing (PYCARD))

Autre désignation: CARD5 / PYCARD / ASC (PYCARD Produits)

Synonymes: anticorps ASC, anticorps CARD5, anticorps TMS, anticorps TMS-1, anticorps TMS1, anticorps 9130417A21Rik, anticorps Asc, anticorps TNS1, anticorps masc, anticorps asc1, anticorps zAsc, anticorps zgc:109874, anticorps PYD and CARD domain containing, anticorps PYCARD, anticorps Pycard, anticorps pycard

Sujet: TMS1 (target of methylation-induced silencing) or ASC (apoptosis-associated speck-like protein containing a CARD) promotes caspase-mediated apoptosis. TMS1 is aberrantly methylated and silenced in human breast cancer cells, the methylation-mediated silencing of TMS1 may confer a survival advantage by allowing cells to escape from apoptosis, supporting a new role for aberrant methylation in breast tumorigenesis. TMS1 encodes a 22- kDa predicted protein containing a COOH-terminal caspase recruitment domain (CARD). However, it is structurally unrelated to other known CARD adaptor and regulatory proteins. Ectopic expression of TMS1 alone can trigger apoptosis in 293 cells, and cell death correlated with relocalization of ASC from the cytoplasm to perinuclear, ball-like structures.Synonyms: Caspase recruitment domain-containing protein 5, PYD and CARD domain-containing protein, TMS1, Target of methylation-induced silencing 1, poptosis-associated speck-like protein containing a CARD

ID gène: 29108

NCBI Accession: NP_037390

UniProt: Q9ULZ3

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome