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ALMS1 anticorps (C-Term)

ALMS1 Reactivité: Humain, Rat, Boeuf (Vache), Chien, Souris WB, IHC (p) Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN374311
  • Antigène Voir toutes ALMS1 Anticorps
    ALMS1 (Alstrom Syndrome 1 (ALMS1))
    Épitope
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    Humain, Rat, Boeuf (Vache), Chien, Souris
    Hôte
    • 11
    • 1
    Chèvre
    Clonalité
    • 11
    Polyclonal
    Conjugué
    • 9
    • 1
    • 1
    • 1
    Cet anticorp ALMS1 est non-conjugé
    Application
    • 7
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Séquence
    C-RVTNQLLGRK VPWD
    Specificité
    This antibody detects ALMS1 at C-term.
    Réactivité croisée (Details)
    Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogène
    Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1
    Top Product
    Discover our top product ALMS1 Anticorps primaire
  • Indications d'application
    Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Antigène
    ALMS1 (Alstrom Syndrome 1 (ALMS1))
    Autre désignation
    ALMS1 (ALMS1 Produits)
    Synonymes
    anticorps ALMS1, anticorps ALSS, anticorps bbb, anticorps ALMS1, centrosome and basal body associated protein, anticorps Alstrom syndrome 1, anticorps putative ALMS1-like protein, anticorps ALMS1, centrosome and basal body associated, anticorps ALMS1, anticorps alms1, anticorps LOC459325, anticorps Alms1
    Sujet
    ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328
    ID gène
    7840
    NCBI Accession
    NP_055935
    UniProt
    Q8TCU4
    Pathways
    Sensory Perception of Sound, Carbohydrate Homeostasis, M Phase
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