AGO1 anticorps (Internal Region)

N° du produit ABIN374655

Détail du produit anti-AGO1 anticorps

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AGO1 est non-conjugé

Application: Western Blotting (WB)

Specificité: This antibody recognizes Protein eIF2C1 /AGO1. This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.3-1 μg/mL. In transfected HEK293 transiently expressing Human EIF2C1bands of approx.110 kDa and 30 kDa are observed. The 110 kDa band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur AGO1

Antigène: AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))

Autre désignation: EIF2C1 / AGO1 (EIF2C1 Produits)

Synonymes: anticorps Ago, anticorps Ago-1, anticorps Ago1, anticorps CG6671, anticorps Dm Ago1, anticorps Dmel\\CG6671, anticorps MRE20, anticorps ago1, anticorps ago1-1, anticorps anon-WO0257455.29, anticorps dAGO1, anticorps dAgo1, anticorps l(2)04845, anticorps l(2)4845, anticorps l(2)k00208, anticorps l(2)k08121, anticorps GB12654, anticorps dsim_GLEANR_9718, anticorps DsimGD25729, anticorps GD25729, anticorps EIF2C1, anticorps EIF2C, anticorps GERP95, anticorps Q99, anticorps Eif2c1, anticorps ARGONAUTE 1, anticorps T1N15.2, anticorps T1N15_2, anticorps Argonaute-1, anticorps protein argonaute-2, anticorps argonaute 1, anticorps Argonaute 1, anticorps protein argonaute-1, anticorps argonaute 1, RISC catalytic component, anticorps argonaute RISC catalytic subunit 1, anticorps Stabilizer of iron transporter SufD / Polynucleotidyl transferase, anticorps AGO1, anticorps LOC552062, anticorps ago1, anticorps LOC659936, anticorps Dsim\AGO1, anticorps Ago1, anticorps LOC100386910, anticorps LOC100482671, anticorps LOC475337

Sujet: Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as cell cycle control protein through it's regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.Synonyms: Argonaute-1, Eukaryotic translation initiation factor 2C1, RNA-binding protein Q99, eIF-2C1, hAgo-1

Poids moléculaire: 97.2kDa according to NP_036331.1

ID gène: 26523

NCBI Accession: NP_036331

UniProt: Q9UL18

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization