GFAP anticorps (C-Term)

N° du produit ABIN374749

Détail du produit anti-GFAP anticorps

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Épitope: C-Term

Reactivité: Humain, Rat, Souris, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFAP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-DGEVIKESKQ EHKD

Specificité: This antibody recognizes GFAP.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Synthetic peptide from the C-Terminus of Human GFAP (NP_002046.1) Genename: GFAP

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/32000. Western Blot: 0.1-0.3 μg/mL. Approx 50 kDa band observed in Human Brain Cerebellum andMouse Brain lysates (calculated MW of 49.0 kDa according to Mouse NP_034407.2 andHuman NP_002046.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur GFAP

Antigène: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Autre désignation: GFAP (GFAP Produits)

Synonymes: anticorps GFAP, anticorps AI836096, anticorps cb345, anticorps etID36982.3, anticorps gfapl, anticorps wu:fb34h11, anticorps wu:fk42c12, anticorps xx:af506734, anticorps zgc:110485, anticorps glial fibrillary acidic protein, anticorps GFAP, anticorps LOC100136168, anticorps gfap, anticorps Gfap

Sujet: Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.Synonyms: Glial Fibrillary Acidic Protein

ID gène: 2670

NCBI Accession: NP_001229305

UniProt: P14136