APPL1 anticorps (Internal Region)

N° du produit ABIN374837

Détail du produit anti-APPL1 anticorps

Antigène: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp APPL1 est non-conjugé

Application: Western Blotting (WB)

Séquence: C-NRYSRLSKKR ENDK

Specificité: This antibody recognizes APPL1.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_036228.1.

Information d'application

Indications d'application: Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in Rat Aortic Smooth SkeletalMuscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for48h (lane 2). Calculated MW of 79.0 kDa according to NP_036228.1. The blot wasco-labeled with Tubulin-specific antibodies as a loading control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur APPL1

Antigène: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Autre désignation: APPL1 / DIP13A (APPL1 Produits)

Synonymes: anticorps APPL, anticorps DIP13alpha, anticorps 2900057D21Rik, anticorps 7330406P05Rik, anticorps AI585782, anticorps AW209077, anticorps BB022931, anticorps C88264, anticorps DIP13, anticorps RGD1309388, anticorps adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1, anticorps adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1, anticorps APPL1, anticorps Appl1

Sujet: APPL is involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The APPL binds to RAB5A/Rab5 through N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation.Synonyms: Adapter protein containing PH domain, DCC-interacting protein 13-alpha, Dip13-alpha, KIAA1428, PTB domain and leucine zipper motif 1

ID gène: 26060, 9606

UniProt: Q9UKG1