DNMT1 anticorps (Internal Region)

N° du produit ABIN375024

Détail du produit anti-DNMT1 anticorps

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-RFESPPKTQP TEDN

Specificité: This antibody recognizes DNMT1.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal egion of the protein sequence Genename: DNMT1

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western Blot: 0.5 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-DNMT1 (ABIN375024)

Dion, Lin, Hubert, Waterland, Wilson: "Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline." dans: Human molecular genetics, Vol. 17, Issue 9, pp. 1306-17, (2008) (PubMed).

Détails sur DNMT1

Antigène: DNMT1 (DNA (Cytosine-5)-Methyltransferase 1 (DNMT1))

Autre désignation: DNMT1 (DNMT1 Produits)

Synonymes: anticorps cb1075, anticorps cb983, anticorps dmnt1, anticorps fb05h01, anticorps mta, anticorps wu:fb05h01, anticorps ADCADN, anticorps AIM, anticorps CXXC9, anticorps DNMT, anticorps HSN1E, anticorps MCMT, anticorps DDM2, anticorps DECREASED DNA METHYLATION 2, anticorps DMT01, anticorps DMT1, anticorps DNA METHYLTRANSFERASE, anticorps DNA METHYLTRANSFERASE 01, anticorps DNA METHYLTRANSFERASE 1, anticorps MET2, anticorps METHYLTRANSFERASE 2, anticorps METHYLTRANSFERASE I, anticorps METI, anticorps methyltransferase 1, anticorps Cxxc9, anticorps Dnmt, anticorps Dnmt1o, anticorps MTase, anticorps Met-1, anticorps Met1, anticorps MommeD2, anticorps m.MmuI, anticorps DNA methyltransferase 1, anticorps DNA (cytosine-5-)-methyltransferase 1, anticorps methyltransferase 1, anticorps DNA methyltransferase (cytosine-5) 1, anticorps DNMT1, anticorps dnmt1, anticorps Dnmt1, anticorps MET1

Sujet: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence specific DNA binding activity, as well as a growth regulatory pathway that is disrupted in nearly all cancer cells.Synonyms: AIM, CXXC-type zinc finger protein 9, CXXC9, DNA (cytosine-5)-methyltransferase 1, DNA methyltransferase HsaI, HsaI, MCMT

Poids moléculaire: 183kDa (NP_001370.1).

ID gène: 1786

NCBI Accession: NP_001124295

UniProt: P26358

Pathways: SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2