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DYKDDDDK Tag anticorps

WB, ELISA, IP Hôte: Souris Monoclonal 5A8E5 unconjugated
N° du produit ABIN387700
  • Antigène Tous les produits DYKDDDDK Tag
    DYKDDDDK Tag
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 57
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    Souris
    Clonalité
    • 66
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    Monoclonal
    Conjugué
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    Cet anticorp DYKDDDDK Tag est non-conjugé
    Application
    • 79
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Marque
    THE™
    Séquence
    DYKDDDDK
    Specificité
    THETM DYKDDDDK Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal and internal tagged fusion proteins.
    Attributs du produit
    Mouse Anti-DYKDDDDK-tag Monoclonal Antibody is a high-affinity monoclonal antibody that can be used to detect DYKDDDDK-tagged proteins.
    Purification
    Protein A affinity column
    Immunogène
    A synthetic peptide (DYKDDDDK) coupled - KLH
    Clone
    5A8E5
    Isotype
    IgG
  • Indications d'application
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
    ELISA: 0.05-0.2 µg/mL
    Western blot: 0.1-1.0 µg/mL
    Immunoprecipitation: 1 µg/mL
    Other applications: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing cycles.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
  • Reuben, Phénix, Santos, Lapointe: "The WT hemochromatosis protein HFE inhibits CD8? T-lymphocyte activation." dans: European journal of immunology, Vol. 44, Issue 6, pp. 1604-14, (2014) (PubMed).

    Maryon, Molloy, Kaplan: "Cellular glutathione plays a key role in copper uptake mediated by human copper transporter 1." dans: American journal of physiology. Cell physiology, Vol. 304, Issue 8, pp. C768-79, (2013) (PubMed).

  • Antigène
    DYKDDDDK Tag
    Abstract
    DYKDDDDK Tag Produits
    Classe de substances
    Tag
    Sujet
    The DYKDDDDK peptide is a small component of the epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors.
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