Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Homologie
C, M, Rat
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogène
This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human Dnmt3a.
DNMT3A
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
RB12504
unconjugated
Indications d'application
WB: 1:1000. IHC-P: 1:10~50
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Date de péremption
6 months
Husni, Shiba-Ishii, Iiyama, Shiozawa, Kim, Nakagawa, Sato, Kano, Minami, Noguchi: "DNMT3a expression pattern and its prognostic value in lung adenocarcinoma." dans: Lung cancer (Amsterdam, Netherlands), Vol. 97, pp. 59-65, (2017) (PubMed).
Chestnut, Chang, Price, Lesuisse, Wong, Martin: "Epigenetic regulation of motor neuron cell death through DNA methylation." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 46, pp. 16619-36, (2011) (PubMed).
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.