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His Tag anticorps

Name: His Tag anticorps
SKU: ABIN387901
Price: 475.5 EUR
Availability: InStock

WB Hôte: Lapin Polyclonal RB19955 unconjugated

Western blot analysis of His Tag Antibody (ABIN387901 and ABIN2843197) in his-tag TBB1 protein (35 μg/lane).

All lanes : Anti-His Tag Antibody at 1:2000 dilution Lane 1: 12-tag recombinant protein lysate Lysates/proteins at 20 μg per lane.

All lanes : Anti-His Tag Antibody at 1:2000 dilution Lane 1: recombinant protein with His tag lysate Lysates/proteins at 20 μg per lane.

3 images

(1 reference)

N° du produit ABIN387901

Fiche de données

Antigène Voir toutes His Tag Anticorps

His Tag

Reactivité

Veuillez nous consulter SVP
Hôte
94

56

12

9

8

7

2

1
Lapin
Anticorps secondaires appropriés
Clonalité
107

69

10
Polyclonal
Conjugué
91

23

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cet anticorp His Tag est non-conjugé
Application
153

103

38

28

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB)

Séquence

HHHHHH

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène

Poly-HIS peptide were used to produced this antibody.

Clone

RB19955

Isotype

IgG

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anti-His Tag antibody
Reactivité: Humain WB Hôte: Lapin Polyclonal RB19960 unconjugated

anti-His Tag (C-Term) antibody
WB, ELISA Hôte: Lapin Polyclonal unconjugated

anti-His Tag antibody (Biotin)
WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2 Biotin

Indications d'application

WB: 1:2000. WB: 1:2000. WB: 1:1000

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Anderson, Robson, Jiang, Malmirchegini, Fierobe, Lazazzera, Clubb: "Assembly of minicellulosomes on the surface of Bacillus subtilis." dans: Applied and environmental microbiology, Vol. 77, Issue 14, pp. 4849-58, (2011) (PubMed).
Antigène

His Tag

Autre désignation

6 X His Tag (His Tag Produits)

Classe de substances

Tag

Sujet

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.