MAP1LC3A anticorps (N-Term)

N° du produit ABIN388464

Détail du produit anti-MAP1LC3A anticorps

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Épitope: AA 1-30, N-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAP1LC3A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).

Clone: RB7609

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:200. WB: 1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-MAP1LC3A (ABIN388464)

Doucet-Beaupré, Gilbert, Profes, Chabrat, Pacelli, Giguère, Rioux, Charest, Deng, Laguna, Ericson, Perlmann, Ang, Cicchetti, Parent, Trudeau, Lévesque: "Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 30, pp. E4387-96, (2018) (PubMed).

Lord, Farrugia, Yan, Vassie, Whitelock: "Hyaluronan coated cerium oxide nanoparticles modulate CD44 and reactive oxygen species expression in human fibroblasts." dans: Journal of biomedical materials research. Part A, Vol. 104, Issue 7, pp. 1736-46, (2017) (PubMed).

Sumiyoshi, Ishitobi, Miyaki, Miyado, Adachi, Ochi: "The role of tetraspanin CD9 in osteoarthritis using three different mouse models." dans: Biomedical research (Tokyo, Japan), Vol. 37, Issue 5, pp. 283-291, (2017) (PubMed).

Paquet, Nicoll, Love, Mouton-Liger, Holmes, Hugon, Boche: "Downregulated apoptosis and autophagy after anti-Aβ immunotherapy in Alzheimer's disease." dans: Brain pathology (Zurich, Switzerland), (2017) (PubMed).

Chen, Akinyemi, Hase, Firbank, Ndungu, Foster, Craggs, Washida, Okamoto, Thomas, Polvikoski, Allan, Oakley, OBrien, Horsburgh, Ihara, Kalaria: "Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia." dans: Brain : a journal of neurology, Vol. 139, Issue Pt 1, pp. 242-58, (2016) (PubMed).

So, Chen, Wang, Wu, Huang, Lai, Pan, Kang, Huang: "Midazolam regulated caspase pathway, endoplasmic reticulum stress, autophagy, and cell cycle to induce apoptosis in MA-10 mouse Leydig tumor cells." dans: OncoTargets and therapy, Vol. 9, pp. 2519-33, (2016) (PubMed).

Castro, Gao, Moore, Yu, Di, Kissling, Dixon: "A High Concentration of Genistein Induces Cell Death in Human Uterine Leiomyoma Cells by Autophagy." dans: Expert opinion on environmental biology, Vol. 5, Issue Suppl 1, (2016) (PubMed).

Takada, Miyaki, Ishitobi, Hirai, Nakasa, Igarashi, Lotz, Ochi: "Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1." dans: Arthritis research & therapy, Vol. 17, pp. 285, (2015) (PubMed).

Zhu, Tong, Teh, Wang: "Forkhead box protein O3 transcription factor negatively regulates autophagy in human cancer cells by inhibiting forkhead box protein O1 expression and cytosolic accumulation." dans: PLoS ONE, Vol. 9, Issue 12, pp. e115087, (2014) (PubMed).

Zhang, Yi, Ko, Kim, Seo, Lee, Lee, Kim: "Expression of LC3 and Beclin 1 in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain." dans: Brain research, Vol. 1519, pp. 31-9, (2013) (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." dans: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, (2011) (PubMed).

Lee, Liao, Tsai, Chiu, Wen: "Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract." dans: Journal of agricultural and food chemistry, Vol. 59, Issue 6, pp. 2347-55, (2011) (PubMed).

Bains, Zaegel, Mize-Berge, Heidenreich: "IGF-I stimulates Rab7-RILP interaction during neuronal autophagy." dans: Neuroscience letters, Vol. 488, Issue 2, pp. 112-7, (2011) (PubMed).

Cirstea, Hideshima, Rodig, Santo, Pozzi, Vallet, Ikeda, Perrone, Gorgun, Patel, Desai, Sportelli, Kapoor, Vali, Mukherjee, Munshi, Anderson, Raje: "Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma." dans: Molecular cancer therapeutics, Vol. 9, Issue 4, pp. 963-75, (2010) (PubMed).

Li, Castano, Hudson, Nowlin, Lin, Bonventre, Swanson, Duffield: "The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 12, pp. 4767-81, (2010) (PubMed).

Wang, Hossain, Tan, Coolman, Zhou, Liu, Casey: "Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth." dans: Oncogene, Vol. 29, Issue 35, pp. 4959-70, (2010) (PubMed).

Hino, Hamada, Tajika, Funayama, Morimura, Sakashita, Yokota, Fukamoto, Mutou, Kobayashi, Yorifuji: "Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells." dans: Journal of electron microscopy, Vol. 59, Issue 6, pp. 495-501, (2010) (PubMed).

Li, Ma, Wang, Liu, Yang, Zhao, Wang, Jin, Zeng, Li, Song, Li, Li, Qian, Cao: "c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3." dans: Cell death and differentiation, Vol. 17, Issue 8, pp. 1277-87, (2010) (PubMed).

Geng, Kohli, Klocke, Roth: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." dans: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, (2010) (PubMed).

Bohensky, Leshinsky, Srinivas, Shapiro: "Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression." dans: Pediatric nephrology (Berlin, Germany), Vol. 25, Issue 4, pp. 633-42, (2010) (PubMed).

Détails sur MAP1LC3A

Antigène: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Autre désignation: LC3 (MAP1LC3A Produits)

Synonymes: anticorps ATG8E, anticorps LC3, anticorps LC3A, anticorps MAP1ALC3, anticorps MAP1BLC3, anticorps 1010001H21Rik, anticorps 4922501H04Rik, anticorps LC3a, anticorps MGC69006, anticorps zgc:77094, anticorps MGC89867, anticorps MAP1LC3A, anticorps map1lc3a, anticorps microtubule associated protein 1 light chain 3 alpha, anticorps microtubule-associated protein 1 light chain 3 alpha, anticorps microtubule associated protein 1 light chain 3 alpha S homeolog, anticorps MAP1LC3A, anticorps Map1lc3a, anticorps map1lc3a.S, anticorps map1lc3a

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Poids moléculaire: 14272

ID gène: 84557

NCBI Accession: NP_115903, NP_852610

UniProt: Q9H492

Pathways: Autophagy