LC3B anticorps (cleaved)

N° du produit ABIN388484

Détail du produit anti-LC3B anticorps

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Épitope: AA 89-122, cleaved

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LC3B est non-conjugé

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Homologie: B

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Clone: RB15839-RB28608

Isotype: Ig Fraction

Information d'application

Indications d'application: IF: 1:100. IF: 1:10~50. ICC: 1:10~50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption: 6 months

Références pour anticorps anti-LC3B (ABIN388484)

Ock, Park, Han, Jeong, Kim, Lee, Hahm: "Genetic ablation or pharmacologic inhibition of autophagy mitigated NSAID-associated gastric damages." dans: Journal of molecular medicine (Berlin, Germany), Vol. 95, Issue 4, pp. 405-416, (2018) (PubMed).

Xu, Huai, Meng, Dong, Liu, Qi, Hu, Fan, Jin, Lv: "L-3-n-Butylphthalide Activates Akt/mTOR Signaling, Inhibits Neuronal Apoptosis and Autophagy and Improves Cognitive Impairment in Mice with Repeated Cerebral Ischemia-Reperfusion Injury." dans: Neurochemical research, Vol. 42, Issue 10, pp. 2968-2981, (2018) (PubMed).

Filipczak, Thomas, Chen, Salzman, McDonald, Lin, Belinsky: "TSC2 Deficiency Unmasks a Novel Necrosis Pathway That Is Suppressed by the RIP1/RIP3/MLKL Signaling Cascade." dans: Cancer research, Vol. 76, Issue 24, pp. 7130-7139, (2017) (PubMed).

Lee, Park, Hahm: "Mitigated NSAID-induced apoptotic and autophagic cell death with Smad7 overexpression." dans: Journal of clinical biochemistry and nutrition, Vol. 60, Issue 1, pp. 55-62, (2017) (PubMed).

Jutten, Keulers, Schaaf, Savelkouls, Theys, Span, Vooijs, Bussink, Rouschop: "EGFR overexpressing cells and tumors are dependent on autophagy for growth and survival." dans: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 108, Issue 3, pp. 479-83, (2013) (PubMed).

Radtke, English, Rondeau, Leib, Lippé, Desjardins: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." dans: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, (2013) (PubMed).

Checinska, Soengas: "The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy." dans: Pigment cell & melanoma research, Vol. 24, Issue 6, pp. 1116-32, (2011) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." dans: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Eby, Rosenbluth, Mays, Marshall, Barton, Sinha, Johnson, Tang, Pietenpol: "ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagy." dans: Molecular cancer, Vol. 9, pp. 95, (2010) (PubMed).

Mitroulis, Kourtzelis, Kambas, Rafail, Chrysanthopoulou, Speletas, Ritis: "Regulation of the autophagic machinery in human neutrophils." dans: European journal of immunology, Vol. 40, Issue 5, pp. 1461-72, (2010) (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." dans: Nature immunology, Vol. 10, Issue 5, pp. 480-7, (2009) (PubMed).

Détails sur LC3B

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation: LC3B (MAP1LC3B Produits)

Synonymes: anticorps ATG8F, anticorps LC3B, anticorps MAP1A/1BLC3, anticorps MAP1LC3B-a, anticorps Map1lc3, anticorps Mpl3, anticorps zbs559, anticorps 1010001C15Rik, anticorps Atg8, anticorps LC3b, anticorps zgc:56434, anticorps wu:fb60g11, anticorps map1lc3b, anticorps MGC76283, anticorps MAP1LC3B, anticorps microtubule associated protein 1 light chain 3 beta, anticorps microtubule-associated protein 1 light chain 3 beta, anticorps microtubule associated protein 1 light chain 3 beta L homeolog, anticorps MAP1LC3B, anticorps Map1lc3b, anticorps map1lc3b, anticorps map1lc3b.L

Sujet: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Poids moléculaire: 14688

ID gène: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy