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Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) anticorps

Détails pour le produit réf. ABIN395003, Fournisseur: Connectez-vous pour afficher
Antigène
  • cstf3
  • MGC76035
  • CSTF3
  • wu:fa99c02
  • wu:fb18b09
  • zgc:56028
  • 4732468G05Rik
  • C79532
  • CstF-77
  • cstf3a
  • CSTF-77
Épitope
AA 1-104
17
15
4
3
3
2
2
2
2
1
1
1
Reactivité
Humain
64
23
23
6
3
2
2
2
1
1
1
Hôte
Souris
50
10
4
Clonalité (Clone)
Monoclonal ()
Conjugué
Inconjugué
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
49
30
11
11
6
6
3
1
Fournisseur
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Supplier Product No.
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Images

Immunogène CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 1D4
Isotype IgG1 kappa
Specificité CSTF3 (AAH09792, 1 a.a. ~ 104 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Autre désignation CSTF3 (CSTF3 Antibody Extrait)
Sujet Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Poids moléculaire 82922 DA
ID gène 12406
Domaine de recherche Cell Structure
Pathways
Indications d'application Western blot = 1:500-1000
Commentaires

Background: The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms.

Restrictions For Research Use only
Buffer PBS with 0.09% (w/v) sodium azide.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock 4 °C/-20 °C
Stockage commentaire Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Date de péremption 6 months
Supplier Images
Western Blotting (WB) image for anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) anticorps (ABIN395003) Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell (antibody concentrati...
Immunohistochemistry (IHC) image for anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) anticorps (ABIN395003) anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 3, 77kDa (CSTF3) (AA 1-104) antibody (Image 2)
Reference: Background Hockert, Yeh, MacDonald: "The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation." dans: The Journal of biological chemistry, Vol. 285, Issue 1, pp. 695-704, 2009 (PubMed).

Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." dans: , Vol. 3, pp. 89, 2007 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." dans: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Taylor, Noguchi, Totoki et al.: "Human chromosome 11 DNA sequence and analysis including novel gene identification. ..." dans: Nature, Vol. 440, Issue 7083, pp. 497-500, 2006 (PubMed).

Kim, Bhinge, Morgan et al.: "Mapping DNA-protein interactions in large genomes by sequence tag analysis of genomic enrichment." dans: Nature methods, Vol. 2, Issue 1, pp. 47-53, 2005 (PubMed).