DYKDDDDK Tag anticorps

N° du produit ABIN400788

Détail du produit anti-DYKDDDDK Tag anticorps

Antigène: DYKDDDDK Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp DYKDDDDK Tag est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Séquence: DYKDDDDK

Attributs du produit: Synonyms: DYKDDDDK Epitope Tag, ECS Epitope Tag, DYKDDDDK-Tag, ECS-tag, D-tag

Purification: Protein A Chromatography followed by extensive dialysis against the buffer.

Immunogène: Synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Remarks: Residues of Glycine and Cysteine were added to the termini to facilitatecoupling.

Clone: 29E4-G7-H11

Isotype: IgG2a

Information d'application

Indications d'application: This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusionproteins. As such, this antibody can be used to identify fusion proteins containing theDYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- orcarboxy- termini of targeted proteins. The epitope tag peptide sequence was first derivedfrom the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions:

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur DYKDDDDK Tag

Antigène: DYKDDDDK Tag

Autre désignation: DYKDDDDK Epitope Tag (DYKDDDDK Tag Produits)

Classe de substances: Tag

Sujet: Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.