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PDCD4 anticorps (pSer457)

PDCD4 Reactivité: Humain, Souris, Rat, Xenopus laevis WB, IHC (p), EIA, IHC (fro) Hôte: Souris Monoclonal 9G6 unconjugated
N° du produit ABIN400797
  • Antigène Voir toutes PDCD4 Anticorps
    PDCD4 (Programmed Cell Death 4 (PDCD4))
    Épitope
    • 15
    • 15
    • 13
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer457
    Reactivité
    • 104
    • 70
    • 60
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    Humain, Souris, Rat, Xenopus laevis
    Hôte
    • 96
    • 6
    • 2
    • 1
    Souris
    Clonalité
    • 92
    • 13
    Monoclonal
    Conjugué
    • 59
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp PDCD4 est non-conjugé
    Application
    • 76
    • 44
    • 36
    • 16
    • 14
    • 14
    • 13
    • 9
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificité
    This antibody is specific for Pdcd4 protein phosphorylated at Ser457. Expect a band approximately 62 kDa in size corresponding to phosphorylated Pdcd4 protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with Human Pdcd4 pS457 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
    Réactivité croisée (Details)
    Species reactivity (expected):Mouse, Rat and Xenopus (100 %).
    Species reactivity (tested):Human.
    Attributs du produit
    Synonyms: H731, Programmed cell death protein 4, Neoplastic transformation inhibitor protein,Nuclear antigen H731-like
    Purification
    Protein A Chromatography
    Clone
    9G6
    Isotype
    IgG1
    Top Product
    Discover our top product PDCD4 Anticorps primaire
  • Indications d'application
    ELISA: 1: 400,000. Western blot: 1: 1,000 - 1: 3,000. Immunohistochemistry on frozen sections: 1.25 - 2.5 μg/mL, we recommend the use of freshfrozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide as preservative
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. To minimize loss of volume (25μl) dilute 1: 10 by adding 225 μl of the buffer statedabove directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect thevolume at the bottom of the vial. Use this intermediate dilution when calculating finaldilutions. Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
    Shelf life: one year from despatch.
    Date de péremption
    12 months
  • Antigène
    PDCD4 (Programmed Cell Death 4 (PDCD4))
    Autre désignation
    PDCD4 (PDCD4 Produits)
    Synonymes
    anticorps H731, anticorps D19Ucla1, anticorps Ma3, anticorps Tis, anticorps Dug, anticorps cb45, anticorps pdcd4, anticorps sb:cb45, anticorps wu:fc84b08, anticorps h731, anticorps MGC69337, anticorps PDCD4, anticorps wu:fa55g03, anticorps wu:fb08a11, anticorps wu:fe01a02, anticorps zgc:66311, anticorps programmed cell death 4, anticorps programmed cell death 4b, anticorps programmed cell death 4 L homeolog, anticorps programmed cell death 4 (neoplastic transformation inhibitor), anticorps programmed cell death 4a, anticorps PDCD4, anticorps Pdcd4, anticorps pdcd4b, anticorps pdcd4.L, anticorps pdcd4, anticorps pdcd4a
    Sujet
    Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)- responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4
    ID gène
    27250
    UniProt
    Q53EL6
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