RFP anticorps (FITC)

N° du produit ABIN401227

Détail du produit anti-RFP anticorps (FITC)

Antigène: RFP (Red Fluorescent Protein (RFP))

Reactivité: Discosoma

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFP est conjugé à/à la FITC

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Réactivité croisée (Details): Ads. to Hu, Ms, Rt Serum Proteins

Purification: Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma

Isotype: IgG

Information d'application

Indications d'application: Flow cytometry: 1: 500 - 1: 2,500. Immunoflourescence: 1: 500 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Commentaires:

Molar Ratio: 2.2 moles FITC per mole of IgG

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (Immunoglobulin and Protease free)

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur RFP

Antigène: RFP (Red Fluorescent Protein (RFP))

Abstract: RFP Produits

Sujet: Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag

ID gène: 86600

UniProt: Q9U6Y8