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RFP anticorps

RFP Reactivité: Discosoma WB, ELISA, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN401229
  • Antigène Voir toutes RFP Anticorps
    RFP (Red Fluorescent Protein (RFP))
    Reactivité
    • 53
    • 1
    Discosoma
    Hôte
    • 23
    • 15
    • 9
    • 5
    • 3
    Lapin
    Clonalité
    • 32
    • 23
    Polyclonal
    Conjugué
    • 21
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RFP est non-conjugé
    Application
    • 29
    • 22
    • 15
    • 12
    • 10
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Réactivité croisée (Details)
    Ads. to Hu, Ms, Rt Serum Proteins
    Purification
    Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogène
    Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
    Isotype
    IgG
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  • Indications d'application
    ELISA: 1: 100,000. Western blot: 1: 2,000 - 1: 5,000. Immunohistochemistry: 1: 200 - 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature.
  • Antigène
    RFP (Red Fluorescent Protein (RFP))
    Abstract
    RFP Produits
    Sujet
    Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
    ID gène
    86600
    UniProt
    Q9U6Y8
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