Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

HA-Tag anticorps

WB, EIA, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN401240
  • Antigène Tous les produits HA-Tag
    HA-Tag
    Reactivité
    Veuillez nous consulter SVP
    Hôte
    • 74
    • 74
    • 13
    • 7
    • 1
    • 1
    Lapin
    Clonalité
    • 85
    • 82
    Polyclonal
    Conjugué
    • 70
    • 15
    • 13
    • 11
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HA-Tag est non-conjugé
    Application
    • 134
    • 62
    • 39
    • 28
    • 19
    • 16
    • 14
    • 14
    • 9
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Séquence
    YPYDVPDYA
    Purification
    Affinity Chromatography
    Immunogène
    Whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (114-122) from Hemagglutinin Influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
    Isotype
    IgG
  • Indications d'application
    Anti-HA is optimally suited for monitoring the expression of HA-tagged fusion proteins. As such, anti-HA/HA can be used to identify fusion proteins containing the HA epitope. Theantibody recognizes the HA epitope tag fused to the amino- or carboxy- termini of targetedproteins, as expressed in many commonly used expression vectors. This antibody has been tested by ELISA and Western blotting against both the immunizingpeptide and HA containing recombinant proteins. This antibody is likely functional forImmunoprecipitation, Immunocytochemistry, and other Immunodetection techniques. Affinity purification of the polyclonal antibody results in very low background levels inassays and low cross-reactivity with other cellular proteins. Recommended Dilutions: ELISA: 1/20,000-1/150,000.
    Restrictions
    For Research Use only
  • Concentration
    0.95 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Antigène
    HA-Tag
    Autre désignation
    HA Epitope Tag (YPYDVPDYA) (HA-Tag Produits)
    Classe de substances
    Tag
    Sujet
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the biochemical properties of the tagged protein. Most often, sequences encoding the epitope tag are included with the target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Vous êtes ici:
Support technique