GLI2 anticorps (Isoform alpha)

N° du produit ABIN401285

Détail du produit anti-GLI2 anticorps

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Épitope: AA 40-60, AA 46-60, Isoform alpha

Reactivité: Humain, Souris, Rat, Chimpanzé, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLI2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against Gli-2 protein. Expect reactivity with all isoforms of this protein.

Réactivité croisée (Details): Species reactivity (expected):Chimpanzee (100 %), Mouse and Rat (93 %), Bovine (86 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogène: Synthetic peptide corresponding to amino acids 46-60 of Human Gli-2 (isoform alpha).

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/2,000-1/12,000. Western blot: 1/500-1/2,000. Detection of Gli-2 may be enhanced if nuclear extracts areused instead of whole cell lysates as the expression/abundance of Gli-2 is likely to be low. Furthermore, Gli-2 expression is likely to be developmentally regulated and induced,making it difficult to detect in whole tissue homogenates. Immunohistochemistry on Paraffin Sections: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur GLI2

Antigène: GLI2 (GLI Family Zinc Finger 2 (GLI2))

Autre désignation: GLI2 (GLI2 Produits)

Synonymes: anticorps HPE9, anticorps THP1, anticorps THP2, anticorps GLI2/GLI4, anticorps GLI3, anticorps AW546128, anticorps GLI family zinc finger 2, anticorps GLI-Kruppel family member GLI2, anticorps GLI2, anticorps Gli2

Sujet: Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli-2 is a nuclear protein thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes, Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. Multiple splice variants have been reported for this protein.Synonyms: GLI-2, THP, Tax helper protein, Zinc finger protein GLI2

ID gène: 2736

NCBI Accession: NP_005261

UniProt: P10070

Pathways: Signalisation Hedgehog, Dopaminergic Neurogenesis