SMAD3
Reactivité: Humain, Souris, Rat
WB, IHC (p), FACS, IF (cc)
Hôte: Lapin
Monoclonal
3D1
unconjugated
Indications d'application
ELISA: 1/15,000 - 1/50,000. Western Blot: 1/500 - 1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.06 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Antigène
SMAD3
(SMAD, Mothers Against DPP Homolog 3 (SMAD3))
anticorps HSPC193, anticorps HsT17436, anticorps JV15-2, anticorps LDS1C, anticorps LDS3, anticorps MADH3, anticorps madh3, anticorps madh3a, anticorps smad3, anticorps wu:fa99e03, anticorps XSmad3, anticorps XenMLP, anticorps Xmad3, anticorps madh3-A, anticorps Madh3, anticorps AU022421, anticorps Smad 3, anticorps mad3, anticorps madh3b, anticorps zgc:92234, anticorps SMAD family member 3, anticorps SMAD family member 3a, anticorps SMAD family member 3 L homeolog, anticorps SMAD family member 3b, anticorps SMAD3, anticorps smad3a, anticorps smad3.L, anticorps Smad3, anticorps smad3b
Sujet
Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type I receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-beta pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway. The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated responses.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3