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SAE1 anticorps (Ser185)

SAE1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Chimpanzé WB, EIA Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN401468
  • Antigène Voir toutes SAE1 Anticorps
    SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
    Épitope
    • 15
    • 11
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser185
    Reactivité
    • 61
    • 41
    • 17
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Chien, Chimpanzé
    Hôte
    • 69
    • 6
    • 1
    Lapin
    Clonalité
    • 62
    • 14
    Polyclonal
    Conjugué
    • 38
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp SAE1 est non-conjugé
    Application
    • 60
    • 27
    • 23
    • 15
    • 14
    • 14
    • 13
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificité
    This antibody is directed against SUMO Activating Enzyme E1 protein.
    Réactivité croisée (Details)
    Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Immunogène
    Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
    Isotype
    IgG
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    Discover our top product SAE1 Anticorps primaire
  • Indications d'application
    ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.71 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    -20 °C
  • Antigène
    SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
    Autre désignation
    SAE1 / AOS1 (SAE1 Produits)
    Synonymes
    anticorps AOS1, anticorps HSPC140, anticorps SUA1, anticorps UBLE1A, anticorps 2400010M20Rik, anticorps 2610044L12Rik, anticorps AL033372, anticorps AW743391, anticorps D7Ertd177e, anticorps Sua1, anticorps Uble1a, anticorps Aos1p, anticorps Sua1p, anticorps aos, anticorps sae2a, anticorps uble1a, anticorps wu:fa28b04, anticorps zgc:86633, anticorps SUMO1 activating enzyme subunit 1, anticorps SUMO1 activating enzyme subunit 1 L homeolog, anticorps SAE1, anticorps Sae1, anticorps sae1.L, anticorps sae1
    Sujet
    SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
    ID gène
    10055
    NCBI Accession
    NP_005491
    UniProt
    Q9UBE0
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