JNK anticorps (pThr183, pTyr185)

N° du produit ABIN401563

Détail du produit anti-JNK anticorps

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Épitope: pThr183, pTyr185

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp JNK est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificité: This antibody detects endogenous levels of JNK1/JNK2 only when phosphorylated at Thr183/Tyr185.

Purification: Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène: The antiserum was produced against synthesized phosphopeptide derived from human JNK1/JNK2 around the phosphorylation site of Thr183/Tyr185 (M-M-Tp-P-YP-V-V).

Information d'application

Indications d'application: Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at-20 °C.

Détails sur JNK

Antigène: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Autre désignation: MAPK8 / JNK1 (MAPK8 Produits)

Synonymes: anticorps JNK, anticorps JNK-46, anticorps JNK1, anticorps JNK1A2, anticorps JNK21B1/2, anticorps PRKM8, anticorps SAPK1, anticorps SAPK1c, anticorps AI849689, anticorps Prkm8, anticorps BSK, anticorps BSK/DJNK, anticorps Bsk, anticorps CG5680, anticorps D-JNK, anticorps D-junk, anticorps DBSK/JNK, anticorps DJNK, anticorps DJNK/bsk, anticorps Dmel\\CG5680, anticorps JNK/SAPK, anticorps Jnk, anticorps Junk, anticorps SAPKa, anticorps c-Jun, anticorps dJNK, anticorps jnk, anticorps T10F20.15, anticorps jnk1, anticorps sapk1, anticorps mapk8, anticorps zgc:112379, anticorps JNKb, anticorps mapk8b, anticorps mitogen-activated protein kinase 8, anticorps Stress-activated protein kinase jnk-1, anticorps basket, anticorps Protein kinase superfamily protein, anticorps mitogen-activated protein kinase 8 L homeolog, anticorps mitogen-activated protein kinase 8b, anticorps mitogen-activated protein kinase 8B-like, anticorps MAPK8, anticorps jnk-1, anticorps Mapk8, anticorps bsk, anticorps ATMPK8, anticorps mapk8.L, anticorps mapk8b, anticorps LOC109094263

Sujet: JNK (c-Jun N-terminal Kinase), also referred to as Stress Activated Protein Kinase (SAPK), is one of the main mitogen-activated protein kinases (MAPKs) in mammals. JNK is expressed as ten different isoforms due to differential mRNA splicing. The predominant forms are JNK1 and JNK2. JNK is activated by a variety of cellular signals including growth factors, inflammatory cytokines, and environmental stress. The JNK/SAPK signaling pathway involves sequential activation of MAPK kinase kinase (MEKK1), MAPK kinase 4 (MKK4) or MKK7, SAPK/JNK, and c-Jun. Full activation of JNK requires phosphorylation of a threonine and a tyrosine residue in the motif Thr-Pro-Tyr. MKK7 and MKK4 phosphorylate JNK at threonine 183 and tyrosine 185, respectively. The JNK pathway functions to modulate cell cycle, apoptotic and transcriptional responses to stress.Synonyms: JNK-46, JNK-55, JNK1/JNK2, MAPK8, MAPK9, PRKM8, PRKM9, SAPK1, c-Jun N-terminal kinase 1, c-Jun N-terminal kinase 2

ID gène: 5599

NCBI Accession: NP_002741

UniProt: P45983

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins