Myc Tag anticorps (HRP)

N° du produit ABIN401754

Détail du produit anti-Myc Tag anticorps (HRP)

Antigène: Myc Tag

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Myc Tag est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Séquence: EQKLISEEDL

Specificité: This antibody is directed against human c-myc.

Attributs du produit: Absorption / Emission: 770 nm / 794 nm
Molar Ratio: 1,8 moles DyLight(TM) 800 per mole of Rabbit IgG

Purification: Affinity chromatography

Immunogène: Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.

Information d'application

Indications d'application: ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/1,000 - 1/5,000.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as perservative

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Myc Tag

Antigène: Myc Tag

Autre désignation: C-Myc Epitope Tag (Myc Tag Produits)

Classe de substances: Tag

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: c-myc tag, myc tag, myc-tag

ID gène: 4609

NCBI Accession: NP_002458

UniProt: P01106