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Recombinant RFP anticorps (Atto 488)

RFP Reactivité: Discosoma IF, ICC, FACS Hôte: Alpaca Monoclonal 2B12-2A1 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
N° du produit ABIN4368335
  • Antigène Voir toutes RFP Anticorps
    RFP (Red Fluorescent Protein (RFP))
    Type d'anticorp
    Recombinant Antibody
    Fragment
    single-domain Antibody (sdAb)
    Reactivité
    • 52
    • 1
    Discosoma
    Hôte
    • 23
    • 15
    • 8
    • 5
    • 3
    Alpaca
    Expression System
    E.coli
    Clonalité
    • 31
    • 23
    Monoclonal
    Conjugué
    • 21
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp RFP est conjugé à/à la Atto 488
    Application
    • 29
    • 21
    • 15
    • 11
    • 9
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Fonction
    Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1
    Specificité
    Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.
    Réactivité croisée (Details)
    Does not cross-react with GFP or mTagBFP derivatives.
    Attributs du produit
    A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
    Purification
    Produced in: E.coli
    Immunogène
    RFP
    Clone
    2B12-2A1
    Labeling Ratio
    A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.
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  • Indications d'application
    Recommended dilution 1:500
    Commentaires

    A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    5 μM
    Buffer
    1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Protect from light!
    Stock
    -20 °C,-80 °C
    Stockage commentaire
    Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
    Date de péremption
    12 months
  • Antigène
    RFP (Red Fluorescent Protein (RFP))
    Autre désignation
    RFP (RFP Produits)
    Poids moléculaire
    26 kDa
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