Smooth Muscle Actin anticorps (N-Term)

N° du produit ABIN452371

Détail du produit anti-Smooth Muscle Actin anticorps

Antigène: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Épitope: N-Term

Reactivité: Humain, Rat, Souris, Poulet, Boeuf (Vache), Cheval

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Smooth Muscle Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000). Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Réactivité croisée (Details): Species reactivity (tested):Human, Bovine, Equine, Rat, Mouse and Chicken.

Purification: Affinity Chromatography on Protein A

Immunogène: Synthetic N- terminus decapeptide of alpha-smooth-muscleisoform of Actin.

Clone: ASM-1

Isotype: IgG2a

Information d'application

Indications d'application: Immunofluorescence MicroscopyImmunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior toantibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluited at 2-8 °CShelf life: One year from despatch.

Date de péremption: 12 months

Détails sur Smooth Muscle Actin

Antigène: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Abstract: ACTA2 Produits

Synonymes: anticorps AAT6, anticorps ACTSA, anticorps MYMY5, anticorps GAMMA-ACTIN, anticorps ACT, anticorps ACTA3, anticorps ACTE, anticorps ACTL3, anticorps ACTSG, anticorps Act-4, anticorps Act4, anticorps SMGA, anticorps 0610041G09Rik, anticorps Actvs, anticorps SMalphaA, anticorps a-SMA, anticorps alphaSMA, anticorps wu:fb63d03, anticorps ASMA, anticorps SMactin, anticorps a-sma, anticorps aat6, anticorps actsa, anticorps mymy5, anticorps sm-actin, anticorps ACTA2, anticorps actc1, anticorps ACT-4, anticorps asma, anticorps ACTGE, anticorps actin, alpha 2, smooth muscle, aorta, anticorps actin, gamma 2, smooth muscle, enteric, anticorps actin, alpha 2, smooth muscle, aorta L homeolog, anticorps ACTA2, anticorps ACTG2, anticorps Actg2, anticorps Acta2, anticorps acta2, anticorps acta2.L

Sujet: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

ID gène: 58

UniProt: P68133

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development