ATF6 anticorps (Middle Region)

N° du produit ABIN453523

Détail du produit anti-ATF6 anticorps

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Épitope: AA 356-386, Middle Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATF6 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes ATF6.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogène: KLH conjugated synthetic peptide between 356~386 amino acids from the Center region of Human ATF6.

Isotype: Ig Fraction

Information d'application

Indications d'application: ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ATF6

Antigène: ATF6 (Activating Transcription Factor 6 (ATF6))

Autre désignation: ATF6 alpha (ATF6 Produits)

Synonymes: anticorps ATF-6-like, anticorps GB16435, anticorps ATF6, anticorps Atf6, anticorps ATF6A, anticorps 9130025P16Rik, anticorps 9630036G24, anticorps AA789574, anticorps Atf6alpha, anticorps ESTM49, anticorps activating transcription factor 6, anticorps uncharacterized LOC412432, anticorps activating transcription factor 6 S homeolog, anticorps cyclic AMP-dependent transcription factor ATF-6 alpha, anticorps ATF6, anticorps LOC412432, anticorps atf6.S, anticorps LOC100122162, anticorps Atf6

Sujet: ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It also interacts with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site 1 and site 2 proteases. ATF6 is N glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.Synonyms: ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha

ID gène: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response